Loading…
In Vitro Potently Active Anti-Plasmodium and Anti-Toxoplasma Mongolian Plant Extracts
Purpose Malaria and toxoplasmosis are important public health diseases affecting millions of people and animals each year, and there is a continuing need for new and improved treatments for them. Plants have provided many opportunities for new drug leads in pharmacology. Methods We examined 43 crude...
Saved in:
Published in: | Acta parasitologica 2021-12, Vol.66 (4), p.1442-1447 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Purpose
Malaria and toxoplasmosis are important public health diseases affecting millions of people and animals each year, and there is a continuing need for new and improved treatments for them. Plants have provided many opportunities for new drug leads in pharmacology.
Methods
We examined 43 crude extracts from Mongolian plants for their activities against the
Plasmodium falciparum
3D7 strain and the
Toxoplasma gondii
RH strain using a SYBR Green-based fluorescence assay and a fluorescence-based assay, respectively. The potential toxicity of these extracts was also assessed on human foreskin fibroblast cells (HFF) using a cell viability assay.
Results
From the initial screenings, 11 and 7 crude extracts were effective against
T. gondii
and
P. falciparum
, respectively, at 100 µg/ml concentration (≥ 80% inhibition activity). The 50% cytotoxic concentrations of the extracts were estimated on HFF cells, and their 50% inhibitory concentrations (IC
50
s) were calculated. According to our lead criteria (selective index, SI; value ≥ 10), six plants (
Galatella dahurica
leaf + flower,
Leonurus deminutus
leaf + flower,
Oxytropis trichophysa
aerial part,
Schultzia crinita
whole plant,
Leontopodium campestre
root,
Spirea salicifolia
aerial part) inhibited
P. falciparum
growth at IC
50
values of 5.99–64.15 µg/ml (SI values: 10.11–17.02).
Amaranthus retroflexus
root was highly active against
T. gondii
(IC
50
, 19.89 µg/ml; SI value, 38).
Conclusion
This first observation of the anti-
Plasmodium
and anti-
Toxoplasma
activities of Mongolian plant extracts shows them to be interesting potential candidates for drug discovery. |
---|---|
ISSN: | 1230-2821 1896-1851 |
DOI: | 10.1007/s11686-021-00401-8 |