Anti-inflammatory activity of citrus pectin on chicken monocytes' immune response

•CP did not affect chicken monocytes’ apoptosis and viability.•CP inhibited the chemotactic activity of chicken monocytes.•CP reduced the phagocytic activity of chicken monocytes.•CP exerts in vitro immunomodulatory effects in poultry. Pectin is a dietary fibre composed of galacturonic acid, primari...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2021-07, Vol.237, p.110269-110269, Article 110269
Main Authors: Ávila, G., De Leonardis, D., Grilli, G., Lecchi, C., Ceciliani, F.
Format: Article
Language:English
Subjects:
Anti-inflammatory
Apoptosis
Chemotaxis
Chicken
Citrus pectin
Monocyte
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Summary:•CP did not affect chicken monocytes’ apoptosis and viability.•CP inhibited the chemotactic activity of chicken monocytes.•CP reduced the phagocytic activity of chicken monocytes.•CP exerts in vitro immunomodulatory effects in poultry. Pectin is a dietary fibre composed of galacturonic acid, primarily found in the citrus fruits' cell walls. Citrus pectin (CP) has demonstrated antioxidative, anticancer, and anti-inflammatory properties in humans and animals. In broilers, CP supplementation improves energy utilization and nutrient digestibility, but limited information on its effects on chicken immunity is available so far. This study aimed to assess the in vitro impact of CP on chicken monocytes' immune response. Cells were purified from whole blood of healthy chickens and incubated with increasing concentrations (0, 0.25, 0.5, 0.75, 1 mg/mL) of CP to determine CP working concentration. The effects of different CP concentrations on cells' apoptosis and viability were assessed by measuring caspase-3 and -7 and the cells' metabolic activity (MTT assay), respectively. CP had no dose-dependent effect on monocyte apoptosis and viability.Then, the effects of CP (0.5 mg/mL) on chicken monocytes' chemotaxis and phagocytosis were assessed by measuring transwell migration and fluorescein-labelled E. coli incorporation, respectively. CP inhibited both monocytes' chemotaxis and phagocytosis.These data demonstrate that CP exerts an immunomodulatory role in chicken monocytes, supporting its integration in nutrition strategies that might be beneficial for the animal's immunity and health.
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2021.110269