l-Histidine-DNA interaction: a strategy for the improvement of the fluorescence signal of poly(adenine) DNA-templated gold nanoclusters

An interesting phenomenon is described that the fluorescence signal of poly(adenine) (A) DNA-templated gold nanoclusters (AuNCs) is greatly improved in the presence of l -histidine by means of l -histidine-DNA interaction. The modified nanoclusters display strong fluorescence emission with excitatio...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2021-06, Vol.188 (6), p.198-198, Article 198
Main Authors: Wang, Hai-Bo, Mao, An-Li, Tao, Bei-Bei, Zhang, Hong-Ding, Xiao, Zhong-Liang, Liu, Yan-Ming
Format: Article
Language:English
Subjects:
Adenine
Analytical Chemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
DNA
DNA - chemistry
DNA - metabolism
Emission
Fluorescence
Fluorescent Dyes - chemistry
Genetic research
Gold
Gold - chemistry
Histidine
Histidine - analysis
Histidine - chemistry
Histidine - metabolism
Imidazole
Imidazoles - analysis
Imidazoles - chemistry
Imidazoles - metabolism
Immobilized Nucleic Acids - chemistry
Immobilized Nucleic Acids - metabolism
Limit of Detection
Metal Nanoparticles - chemistry
Microengineering
Nanochemistry
Nanoclusters
Nanotechnology
Original Paper
Poly A - chemistry
Poly A - metabolism
Purines
Spectrometry, Fluorescence
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Summary:An interesting phenomenon is described that the fluorescence signal of poly(adenine) (A) DNA-templated gold nanoclusters (AuNCs) is greatly improved in the presence of l -histidine by means of l -histidine-DNA interaction. The modified nanoclusters display strong fluorescence emission with excitation/emission maxima at 290/475 nm. The fluorescence quantum yield (QY) is improved from 1.9 to 6.5%. Fluorescence enhancement is mainly ascribed to the l -histidine-DNA interaction leading to conformational changes of the poly(A) DNA template, which offer a better microenvironment to protect AuNCs. The assay enables l -histidine to be determined with good sensitivity and a linear response that covers the 1 to 50 nM  l -histidine concentration range with a 0.3 nM limit of detection. The proposed method has been applied to the determination of imidazole-containing drugs in pharmaceutical samples. Graphical abstract A turn-on fluorescent method has been designed for the sensitive detection of l -histidine as well as imidazole-containing drugs on the basis of the l -histidine-DNA interaction.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-021-04853-7