First report of Spirocerca lupi larva in dung beetles (Scarabaeus armeniacus) in the central region of Iran: A morphological and molecular identification

• Published in: Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2021-08, Vol.77, p.101671-101671, Article 101671
• Main Authors: Mohtasebi, Sina, Teimouri, Aref, Abbaszadeh Afshar, Mohammad Javad, Mobedi, Iraj, Abbasian, Hamed, Totonchian, Niloofar, Mowlavi, Gholamreza
• Format: Article
• Language: English
• Subjects: Beetles; Coleoptera; cox1; Cytochrome; Cytochrome-c oxidase; Cytochromes; Detritivores; Dung; Dung beetles; Insects; Iran; Larvae; Mitochondria; Morphology; Phylogeny; Physical characteristics; Rural areas; Scarabaeus; Scarabaeus armeniacus; Spirocerca lupi; Vertebrates
• ISSN: 0147-9571; 1878-1667
• DOI: 10.1016/j.cimid.2021.101671

•First study to assess infections of dung beetles with larval stages of helminths in rural areas of Alborz Province, Iran.•Spirocerca lupi 3rd larval stages were recovered from three female Scarabaeus armeniacus beetles.•First record of Spirocerca lupi 3rd larval stages in Scarabaeus armeniacus from Iran.•S. armeniacus was introduced as a potentially biological vector of S. lupi for the first time. Dung beetles as detritivores insects, naturally use feces of vertebrates as foods and reproduction beds. This leads to frequent contacts between dung beetles and parasitic helminths. The current study was carried out to assess infections of dung beetles with larval stages of helminths in rural areas of Taleqan County, Alborz Province, Iran. A total number of 200 dung beetles were collected randomly in June 2017 from the highlands of Taleqan County. Beetles were dissected in normal saline and carefully studied using a stereomicroscope. Morphological characteristics of the recovered larvae were drawn using a camera lucida equipped microscope at 400× magnification. Then, genomic DNAs of the recovered larvae extracted and PCR amplifications of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene were carried out and the amplicons were sequenced. All collected dung beetles identified as Scarabaeus armeniacus from the Scarabaeidae family (55.5 % were male and 44.5 % female). Three females of the beetles have been found to be naturally infected with the third stage larvae of Spirocerca lupi. The average length and width of the larvae were 2.95 (2.81–3.15; CI 95 %) and 0.12 (0.1–0.15; CI 95 %) mm, respectively. The phylogenetic analysis showed that S. lupi belonged to a clade within the Spirocercidae family. In the current study, S. armeniacus introduced as a potential biological vector for transmission of S. lupi to vertebrates in the region. To the best of the authors’ knowledge, this is the first report on the larval stages of S. lupi in S. armeniacus.