Fluorescent hollow ZrO2@CdTe nanoparticles-based lateral flow assay for simultaneous detection of C-reactive protein and troponin T

• Published in: Mikrochimica acta (1966) 2021-06, Vol.188 (6), p.209-209, Article 209
• Main Authors: Liu, Xin, Ren, Xiangling, Chen, Lufeng, Zou, Jian, Li, Ting, Tan, Longfei, Fu, Changhui, Wu, Qiong, Li, Chaohui, Wang, Jiayi, Huang, Zhongbing, Meng, Xianwei
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Antigens; Assaying; Biomarkers; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Core-shell structure; Fluorescence; Microengineering; Nanochemistry; Nanoparticles; Nanotechnology; Original Paper; Proteins; Zirconium dioxide
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-021-04865-3

Highly fluorescent hollow ZrO 2 @CdTe nanoparticles (NPs) were synthesized efficiently via the hydrothermal method. By changing the hydrothermal time of ZrO 2 @CdTe NP, the peaks of fluorescence spectra measured at fluorescent excitation of 330 nm were at 540 nm, 590 nm, and 640 nm, respectively. Hollow ZrO 2 NPs have a uniform core–shell structure with the size of 178 ± 10 nm and shell of 19 ± 4 nm. The as-prepared yellow-ZrO 2 @CdTe NPs were used to develop lateral flow assay (LFA) for the sensitive and qualitative detection of C-reactive protein (CRP). The visual limit of detection of the LFA for the CRP antigen was 1 μg/L within 20 min, which is 1000-fold lower than that of colloidal gold-based LFA. In addition, a multiplex lateral flow assay (mLFA) was developed using the as-prepared green and red-ZrO 2 @CdTe NPs for the simultaneous, specific, sensitive, and qualitative detection of CRP and troponin T (cTnT). The visual limits of detection of CRP and cTnT in mLFA were 10 μg/L and 0.1 mg/L, respectively. The excellent performance of ZrO 2 @CdTe NPs should facilitate their application in point-of-care technology for the detection of other biomarkers. Graphical abstract