Patient‐specific establishment of bacterial composition within the peri‐implant microbiota during the earliest weeks after implant uncovering

Background and Objective Dysbiosis, a loss of balance in the microbiota, is a potential factor of peri‐implantitis. However, compositional change of the peri‐implant microbiota soon after implant uncovering is still unknown. In this study, bacterial composition in the peri‐implant sulcus was examine...

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Bibliographic Details
Published in:Journal of periodontal research 2021-10, Vol.56 (5), p.964-971
Main Authors: Shimogishi, Masahiro, Watanabe, Takayasu, Shibasaki, Masaki, Shiba, Takahiko, Komatsu, Keiji, Nemoto, Takashi, Ishihara, Kazuyuki, Nakano, Yoshio, Iwata, Takanori, Kasugai, Shohei, Nakagawa, Ichiro
Format: Article
Language:English
Subjects:
Bacteria
bacterial composition
Dental implants
Dentistry
Diversity indices
Dysbacteriosis
Microbiota
Patients
peri‐implant sulcus
rRNA 16S
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Summary:Background and Objective Dysbiosis, a loss of balance in the microbiota, is a potential factor of peri‐implantitis. However, compositional change of the peri‐implant microbiota soon after implant uncovering is still unknown. In this study, bacterial composition in the peri‐implant sulcus was examined to understand the establishment of bacterial composition within the peri‐implant microbiota during the earliest weeks after implant uncovering. Methods Microbiota samples were collected at weeks 1, 2, 4, and 6 after stage‐two surgery. Bacterial DNA was isolated from the samples, and a 16S rRNA gene library was constructed. Sequence reads were obtained using a high‐throughput sequencing platform and were taxonomically assigned at the phylum and genus levels. Results Alpha diversity indices, which did not include taxonomic information, were at similar levels throughout the four time points. At 1 and 2 weeks, the bacterial composition was similar among patients with the predominance of Firmicutes and Proteobacteria. However, the composition was diverse at 4 and 6 weeks and significantly dissimilar to the composition at 1 week. Conclusions At 1 week, the peri‐implant microbiota was already formed with alpha diversity as high as that at the later time points. However, the bacterial composition was not highly dissimilar among patients at 1 week. The composition changed over the passage of several weeks and was specific for each patient.
ISSN:0022-3484
1600-0765
DOI:10.1111/jre.12898