In vitro dentine tubule occlusion by a novel toothpaste containing calcium silicate and sodium phosphate

To investigate the deposition, formation of hydroxyapatite (HAP) and acid resistance of dentine surfaces following brushing with a toothpaste containing calcium silicate and sodium phosphate (CSSP) and fluoride in vitro. Human dentine specimens were brushed with a slurry of CSSP toothpaste followed...

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Bibliographic Details
Published in:Journal of dentistry 2020-01, Vol.103, p.100024-100024, Article 100024
Main Authors: Li, Haoze, Liu, Weining, Zhou, Huan-Jun, Sun, Yuekui, Zhang, Meili, Wang, Jinfang, Limer, Adam, Owens, Gareth, Joiner, Andrew
Format: Article
Language:English
Subjects:
Acid resistance
Acids
Brushing
Calcium fluoride
Calcium phosphates
Calcium silicate
Calcium silicates
Citric acid
Dentifrices
Dentine
Dentistry
Deposition
Electron diffraction
Enamel
Exposure
Fluoride treatments
Fluorides
Hydroxyapatite
Hypersensitivity
In vitro methods and tests
Occlusion
Pain
Scanning electron microscopy
Silica
Silicon dioxide
Slurries
Sodium phosphate
Toothpaste
Transmission electron microscopy
Tubules
X-ray spectroscopy
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Summary:To investigate the deposition, formation of hydroxyapatite (HAP) and acid resistance of dentine surfaces following brushing with a toothpaste containing calcium silicate and sodium phosphate (CSSP) and fluoride in vitro. Human dentine specimens were brushed with a slurry of CSSP toothpaste followed by exposure to simulated oral fluid (SOF) in two in vitro studies, with a silica-based non-occluding toothpaste as control. The surface and tubule deposits were analysed after 14 cycles with scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDX), transmission electron microscopy (TEM) and selected area electron diffraction (SAED). In a third study, dentine specimens were additionally exposed to citric acid erosive challenges for 30, 300 or 600 s after 2, 6, 10 and 14 cycles of SOF and either the CSSP toothpaste or a positive control toothpaste containing calcium sodium phosphosilicate and fluoride. The level of tubule occlusion was evaluated using SEM. The SEM analyses indicated complete coverage of the dentine surface following 14 cycles of brushing with CSSP toothpaste with no observable patent tubules, in contrast to the non-occluding control toothpaste. The TEM and SAED analyses confirmed the deposited material on the surface and within tubules was HAP. The deposited material from CSSP toothpaste was more acid resistant than the deposited material from the positive control toothpaste at all time points and acid exposure levels (p 
ISSN:0300-5712
1879-176X
DOI:10.1016/j.jjodo.2020.100024