Improvement of saccharide yield from wood by simultaneous enzymatic delignification and saccharification using a ligninolytic enzyme and cellulase

White-rot fungi are thought to hold promise for development of a delignification pretreatment process for wood biorefinery that is less energy-consuming than current processes. However, the reaction must take place over weeks and consumes non-neglectable amounts of saccharides. To establish a biolog...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2021-09, Vol.132 (3), p.213-219
Main Authors: Mori, Toshio, Ikeda, Kohei, Kawagishi, Hirokazu, Hirai, Hirofumi
Format: Article
Language:English
Subjects:
Co-oxidants
In vitro degradation
Lignin
Manganese peroxidase
Phanerochaete sordida YK-624
Saccharification
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Summary:White-rot fungi are thought to hold promise for development of a delignification pretreatment process for wood biorefinery that is less energy-consuming than current processes. However, the reaction must take place over weeks and consumes non-neglectable amounts of saccharides. To establish a biological process for wood biorefinery would first require establishment of an enzymatic approach to delignification. Such an approach has the potential to lower costs and reduce saccharide loss. Here, we attempted enzymatic delignification reactions using manganese peroxidases (MnP), a lignin-degrading enzyme, under several reaction conditions. The delignification rate from beech wood meal (particle size
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2021.04.016