Assessment of metabolic activity of human cells in solution and in polymer matrix with the use of metabolite-sensitive sensors

We developed metabolite-sensitive electrochemical sensors on the basis of electrodes modified with a thick film of carbon nanotubes. Modified electrodes provide efficient pre-adsorption of cellular metabolites and their sensitive detection using anodic square-wave voltammetry. On the electrode surfa...

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Bibliographic Details
Published in:Materials Science & Engineering C 2012-10, Vol.32 (7), p.1843-1848
Main Authors: Saifullina, Diana V., Shakhmaeva, Irina I., Abdullin, Timur I.
Format: Article
Language:English
Subjects:
Antioxidants
Apoptosis
Assessments
ATP
Biomaterials
Cellular
Electrochemical (bio-)sensors
Electrodes
Human
Human cells
Metabolites
Nucleotides
Sensors
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Summary:We developed metabolite-sensitive electrochemical sensors on the basis of electrodes modified with a thick film of carbon nanotubes. Modified electrodes provide efficient pre-adsorption of cellular metabolites and their sensitive detection using anodic square-wave voltammetry. On the electrode surface both adhered and non-adhered human cells produce three oxidation peaks at the potentials of +0.82, +1.05, and +1.17V attributed to three groups of cellular metabolites: amino acid-derived antioxidants including glutathione, guanine nucleotides, and also adenine nucleotides including ATP. The electrochemical response was well correlated with cell viability, intracellular ATP level and induction of apoptosis, as determined by independent assays. Developed sensors allow for robust and cost-effective assessment of ATP in cells in contrast to enzyme-based electrodes and conventional bioluminescent assay. Results can be used for rapid analysis of human cells for the purpose of medical diagnostics, transplantology, and toxicological screening. Additionally, we combined modified electrodes with human cells entrapped in agarose matrix. The resulting biosensor allowed for electrochemical monitoring of metabolic activity and death of cells within polymeric matrix that is of interest for tissue engineering applications. ► We developed effective sensors for adsorption and detection of cellular metabolites. ► Sensors provide enzyme-free assessment of ATP content and viability for human cells. ► We designed new biosensor for monitoring of metabolic activity of encapsulated cells.
ISSN:0928-4931
1873-0191
DOI:10.1016/j.msec.2012.05.001