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Biofabrication and characterization of cyanobacteria derived ZnO NPs for their bioactivity comparison with commercial chemically synthesized nanoparticles

[Display omitted] •Screening of 30 cyanobacterial extracts was done for the biosynthesis of ZnONPs.•As per SEM data Oscillatoria sp. synthesized smallest ZnONPs (~40 nm) in 180 min.•pH, temp., reaction time, concentration of extract & metal precursor was optimized.•UV–Vis, FTIR, XRD, DLS, SEM–ED...

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Published in:Bioorganic chemistry 2021-08, Vol.113, p.104999-104999, Article 104999
Main Authors: Asif, Nida, Fatima, Samreen, Aziz, Md. Nafe, Shehzadi, Zaki, Almaz, Fatma, Tasneem
Format: Article
Language:English
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Summary:[Display omitted] •Screening of 30 cyanobacterial extracts was done for the biosynthesis of ZnONPs.•As per SEM data Oscillatoria sp. synthesized smallest ZnONPs (~40 nm) in 180 min.•pH, temp., reaction time, concentration of extract & metal precursor was optimized.•UV–Vis, FTIR, XRD, DLS, SEM–EDAX, TEM-SAED & Zeta potential techniques were used.•Biogenic ZnO NPs showed higher bioactivity than chemically synthesized NPs. Due to unique properties of the nanoparticles (NPs) with biocompatibility, their application as drug in drug delievery and diagnostics, the recent scientific branch nanotechnology has emerged as hope in modern medicine. Zinc oxide nanoparticles (ZnO NPs) have gained tremendous interest due to their potential use as chemotherapeutic and antimicrobial agents. They are included in the category of “generally recognized as safe (GRAS) metal oxide”. There is an urgent need for developing additional sources of ZnO NPs. Therefore, in the present study 30 cyanobacterial extracts were screened for ZnO NPs synthesis.. The color change of the reaction mixture from blue to pale white indicated the synthesis of ZnO NPs. It was further confirmed by UV–Visible spectroscopy that showed the absorption peak at 372 nm. The SEM analysis during screening revealed that Oscillatoria sp. synthesized smallest ZnO NPs (~40 nm) that were further optimized for their higher yield by altering reaction conditions (pH, temperature, reaction time, concentration of extract and metal precursor). Best conditions for ZnO NPs synthesis are (0.02 M zinc nitrate, 10 ml of extract volume, pH 8, at 80 °C for 3 h). The NPs were purified through calcination at 350°C and characterized by UV–Vis, FTIR, XRD, SEM-EDAX, TEM, Zeta potential and DLS analysis. The comparative analysis of purified biogenic ZnO NPs with commercial chemically synthesized ZnO NPs (CS), exhibited their superior nature as antioxidant and anti-bacterial agent against both gram-positive and gram-negative bacteria. Synergistic effects of biogenic ZnO NPs and streptomycin additionally favored for their future use as a potential biomedical agent.
ISSN:0045-2068
1090-2120
DOI:10.1016/j.bioorg.2021.104999