Dynamics of small non-coding RNAs in bovine scNT embryos through the maternal-to-embryonic transition

The efficiency of somatic cell nuclear transfer (scNT) for production of viable offspring is relatively low as compared to in vitro fertilization (IVF), presumably due to deficiencies in epigenetic reprogramming of the donor cell genome. Such defects may also involve the population of small non-codi...

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Bibliographic Details
Published in:Biology of reproduction 2021-10, Vol.105 (4), p.918-933
Main Authors: Cuthbert, Jocelyn M, Russell, Stewart J, Polejaeva, Irina A, Meng, Qinggang, White, Kenneth L, Benninghoff, Abby D
Format: Article
Language:English
Subjects:
Analysis
Animals
Cattle - embryology
Cell differentiation
Developmental stages
Embryo, Mammalian - metabolism
Embryogenesis
Embryonic development
Embryonic Development - genetics
Epigenetic inheritance
Epigenetics
Genomes
Genomics
In vitro fertilization
maternal-to-embryonic transition
MicroRNA
miRNA
Non-coding RNA
Nuclear transfer
Nuclear Transfer Techniques
piRNA
RESEARCH ARTICLE
RNA sequencing
RNA, Small Untranslated - metabolism
Sequence analysis
small non-coding RNA
Somatic cell nuclear transfer
Transfer RNA
tRNA
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Summary:The efficiency of somatic cell nuclear transfer (scNT) for production of viable offspring is relatively low as compared to in vitro fertilization (IVF), presumably due to deficiencies in epigenetic reprogramming of the donor cell genome. Such defects may also involve the population of small non-coding RNAs (sncRNAs), which are important during early embryonic development. The objective of this study was to examine dynamic changes in relative abundance of sncRNAs during the maternal-to-embryonic transition (MET) in bovine embryos produced by scNT as compared to IVF by using RNA sequencing. When comparing populations of miRNA in scNT versus IVF embryos, only miR-2340, miR-345, and miR34a were differentially expressed in morulae, though many more miRNAs were differentially expressed when comparing across developmental stages. Also of interest, distinct populations of piwi-interacting like RNAs (pilRNAs) were identified in bovine embryos prior to and during embryonic genome activation (EGA) as compared bovine embryos post-EGA and differentiated cells. Overall, sncRNA sequencing analysis of preimplantation embryos revealed largely similar profiles of sncRNAs for IVF and scNT embryos at the 2-cell, 8-cell, morula, and blastocyst stages of development. However, these sncRNA profiles, including miRNA, piRNA, and tRNA fragments, were notably distinct prior to and after completion of the MET. Summary sentence Small non-coding RNA sequencing analysis revealed largely similar sncRNA for IVF and scNT embryos at the 2-cell, 8-cell, morula and blastocyst stages of development. Graphical Abstract
ISSN:0006-3363
1529-7268
DOI:10.1093/biolre/ioab107