Evaluation of the influence of temperature on the protein-tannic acid complex

Precipitation of blood products from plasma fractionation has played a fundamental role in the industrial purification of important therapeutic products. Only a few studies have been reported by using tannins as proteins precipitant agent from whole plasma while, several conditions have been analyze...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2021-07, Vol.182, p.2056-2065
Main Authors: Nascimento, Jéssica Miranda do, Leão, Talita Emanuely Henrique, Nascimento, Thiago Pajeú, Conniff, Amanda Sales, Batista, Juanize Matias da Silva, Costa, Romero Marcos Pedrosa Brandão, Porto, Ana Lúcia Figueiredo, Leite, Ana Cristina Lima
Format: Article
Language:English
Subjects:
Human plasma
Plasma Proteins
Protein precipitation
Tannic acid
Thermoprotection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Precipitation of blood products from plasma fractionation has played a fundamental role in the industrial purification of important therapeutic products. Only a few studies have been reported by using tannins as proteins precipitant agent from whole plasma while, several conditions have been analyzed. Here, we decided to verify the effect of the temperature on the precipitation process of plasma proteins using tannic acid (TA). Plasma proteins were precipitated with tannic acid by using different temperature incubations. Subsequently, the protein-TA complex was analyzed by SDS-PAGE and quantified. In addition, the protein activity of the complex was measured after heating, as well as the structural changes of the complexes were accompanied by thermogravimetric analysis, differential scanning calorimetry and circular dichroism. In all conditions tested, tannic acid was able to precipitate without selectively separating the proteins in the mixture by using different temperatures during the precipitation process. Furthermore, the protein concentration from the plasma precipitate was not affected by different temperatures and the plasma precipitate was able to dissolve fibrin clots in vitro. [Display omitted]
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2021.05.179