Exercise and pyridostigmine prevents gastric emptying delay and increase blood pressure and cisplatin-induced baroreflex sensitivity in rats

• Published in: Life sciences (1973) 2021-02, Vol.267, p.118972, Article 118972
• Main Authors: Silva, Mariana Sousa, de Andrade Gomes, Yasmim, de Sousa Cavalcante, Mickael Laudrup, Telles, Pedro Victor Nogueira, da Silva, Alda Cássia Alves, Severo, Juliana Soares, de Oliveira Santos, Raisa, dos Santos, Brenda Lois Barros, Cavalcante, Gisele Lopes, Rocha, Cláudio Henrique Lima, Palheta-Junior, Raimundo Campos, de Cássia Meneses Oliveira, Rita, dos Santos, Rosimeire Ferreira, Sabino, João Paulo Jacob, dos Santos, Armenio Aguiar, da Silva, Moisés Tolentino Bento
• Format: Article
• Language: English
• Subjects: Animals; Autonomic nervous system; Autonomic Nervous System - physiopathology; Baroreceptors; Baroreflex; Baroreflex - drug effects; Baroreflex - physiology; Blood pressure; Blood Pressure - drug effects; Blood Pressure - physiology; Cardiovascular System - physiopathology; Cisplatin; Cisplatin - adverse effects; Cisplatin - pharmacology; Colorimetry; Contraction; Exercise; Gastric dysmotility; Gastric emptying; Gastric Emptying - drug effects; Gastric Emptying - physiology; gastrointestinal system; Heart - drug effects; Heart rate; Heart Rate - drug effects; Hemodynamics; Male; Myocardial Infarction - physiopathology; Parameter sensitivity; Parasympathetic nervous system; phenol; Phenols; Physical Conditioning, Animal - physiology; Physical exercise; Physical training; Pyridostigmine; Pyridostigmine Bromide - pharmacology; Rats; Rats, Wistar; Reflexes; Sensitivity analysis; Swimming; Sympathetic nervous system; Vagus nerve; Vagus Nerve - drug effects
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2020.118972

Cisplatin treatment induces an autonomic dysfunction and gastrointestinal and cardiovascular disorders. Physical exercise as well as pyridostigmine treatment induces improves in the autonomic nervous system. In the current study, we investigated the effect of physical exercise and pyridostigmine treatment on gastrointestinal and cardiovascular changes in cisplatin-treated rats. Rats were divided into groups: Saline (S), Cisplatin (Cis), Exercise (Ex), Cisplatin+Exercise (Cis+Ex), Pyridostigmine (Pyr), and Cisplatin+Pyridostigmine (Cis+Pyr). We induced gastrointestinal dysmotility by administering 3 mg kg−1 of cisplatin once week for 5 weeks. The Ex was swimming (1 h per day/5 days per week for 5 weeks with 5% b.w.). GE was evaluated through the colorimetric method of fractional red phenol recovery 10 min after feeding. Pyr groups received 1.5 mg kg−1, p.o. or concomitant Cis treatment. Moreover, gastric contraction in vitro and hemodynamic parameters such as MAP, HR, and evoked baroreflex sensitivity were assessed, as well as sympathetic and parasympathetic tone and intrinsic heart rate (IHR). Cis decrease GE vs. saline (p