Escherichia coli Dcu C4-dicarboxylate transporters dependent proton and potassium fluxes and FOF1-ATPase activity during glucose fermentation at pH 7.5

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2021-10, Vol.141, p.107867-107867, Article 107867
• Main Authors: Karapetyan, L., Mikoyan, G., Vassilian, A., Valle, A., Bolivar, J., Trchounian, A., Trchounian, K.
• Format: Article
• Language: English
• Subjects: dcuD gene expression; Dicyclohexylcarbodiimide; E coli; Efflux; Escherichia coli; Fermentation; FOF1-ATPase; Gene expression; Glucose fermentation; Membrane potential; Membranes; Mutants; pH effects; Potassium; Proton and potassium transport; Protons; Succinate efflux
• ISSN: 1567-5394; 1878-562X
• DOI: 10.1016/j.bioelechem.2021.107867

•DcuD dependent FOF1-ATPase activity was shown.•DCCD-sensitive proton fluxes were absent in dcuD mutant.•Potassium uptake was absent in dcuD and stimulated in dcuACB mutants.•Increased membrane potential was observed in dcu mutants.•dcuD gene expression increased in the dcuACB mutant respect to wild type at pH 7.5 ~ 120%. During fermentation in Escherichia coli succinate is transported via Dcu transporters, encoded dcuA, dcuB, dcuC and dcuD although the role of DcuD protein has not been elucidated yet. It has been shown contribution of Dcu transporters in the N,N’-dicyclohexylcarbodiimide (DCCD) sensitive proton and potassium transport through the cytoplasmic membrane and membrane-associated ATPase activity. Total H± efflux was decreased ~ 40% while K± uptake was absent in dcuD mutant. DCCD-sensitive H± flux was absent in dcuD nevertheless it was increased ~ 3 fold in dcuACB. K± uptake in dcuACB was stimulated ~ 30% compared to wild type but in DCCD assays K± ions were effluxed with the rate of 0.15 mmol/min per 109 cells/ml. In dcuACB mutant membrane potential (ΔΨ) was ~ 30 mV higher than in wild type. dcuD gene expression was increased in the dcuACB mutant respect to wild type at pH 7.5 (~120%), suggesting that an increment of DcuD activity compensates the lack of DcuA, DcuC and DcuB carriers. It can be concluded that active DcuD is important for H± efflux via the FOF1-ATPase and K± uptake at pH 7.5. In addition, DcuA, DcuB and DcuC transporters are crucial for regulating DCCD-sensitive K± transport and ΔΨ in E. coli.