Calorie restriction conferred improvement effect on long-term rehabilitation of ischemic stroke via gut microbiota

• Published in: Pharmacological research 2021-08, Vol.170, p.105726-105726, Article 105726
• Main Authors: Huang, Jia-Ting, Mao, Yu-Qin, Han, Bing, Zhang, Zheng-Yan, Chen, Hui-Ling, Li, Zhan-Ming, Kong, Chao-Yue, Xu, Jia-Qi, Cai, Pei-Ran, Zeng, Yu-Peng, Zhao, Jing, Zhao, Yan-Ping, Wang, Li-Shun
• Format: Article
• Language: English
• Subjects: Animals; Bacteria - growth & development; Bacteria - metabolism; Brain - metabolism; Brain - physiopathology; Brain-Gut Axis; Caloric Restriction; Calorie restriction; Disease Models, Animal; Dysbiosis; Gastrointestinal Microbiome; Gut microbiota; Ischemic stroke; Ischemic Stroke - metabolism; Ischemic Stroke - microbiology; Ischemic Stroke - physiopathology; Ischemic Stroke - rehabilitation; Mice; Recovery of Function; Rehabilitation; Stroke Rehabilitation; Time Factors
• ISSN: 1043-6618; 1096-1186
• DOI: 10.1016/j.phrs.2021.105726

Calorie restriction can modulate the gut microbiota and protect against many diseases including ischemic stroke. However, the role of calorie-restriction-induced microbiota alteration remained unknown in ischemic stroke rehabilitation. Here we conducted 30% reduction of caloric intake on mice for four weeks, to evaluate its role on ischemic stroke rehabilitation. Significantly, this calorie restriction led to better long-term rehabilitation in comparison of normal control. Notably, the transplantation of gut microbiome from calorie-restriction-treated mice to post-stroke mice was eligible to obtain better long-term rehabilitation of stroke mice. Bifidobacterium identified by 16 S ribosomal RNA sequencing were enriched in those of calorie-restriction mice. Then we administrated Bifidobacterium to stroke mice and found Bifidobacterium treatment could successfully improve the long-term rehabilitation of cerebral ischemia mice. Furthermore, the metabolomics analysis revealed a panel of upshifting metabolites, suggesting that calorie restriction greatly altered the gut microbiota composition and its metabolism. Hence, we discovered the novel effect of CR on long-term rehabilitation of ischemic stroke and the underlying role of gut microbiota, which might provide novel thoughts for the clinical post-stroke rehabilitation. Calorie restriction confers protective effects on long-term rehabilitation of stroke, partly through gut microbiota modulation, especially by enrichment of Bifidobacterium. [Display omitted] •Calorie restriction greatly alters the composition of gut microbiota for mice to confer protective effects on ischemic stroke.•Calorie restriction greatly influences the holistic metabolism profile, which is associated with the rehabilitation.•Upshifting the abundance of Bifidobacterium enables to improve the rehabilitation of post-stroke mice.