Homeobox gene amplification and methylation in oral squamous cell carcinoma

•Homeobox genes HOXA9, HOXB13 and IRX4 are amplified in oral squamous cell carcinoma.•HOXD10 was hypermethyladed in oral squamous cell carcinoma.•Genetic alterations may contribute to deregulated expression of homeobox genes. Investigate the DNA copy number and the methylation profile of the homeobo...

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Bibliographic Details
Published in:Archives of oral biology 2021-09, Vol.129, p.105195-105195, Article 105195
Main Authors: Rodrigues, Maria Fernanda Setúbal Destro, Xavier, Flávia Caló Aquino, Esteves, Carina Duarte, Nascimento, Rebeca Barros, Nobile, Juliana Stephan, Severino, Patrícia, de Cicco, Rafael, Toporcov, Tatiana Natasha, Tajara, Eloiza Helena, Nunes, Fábio Daumas
Format: Article
Language:English
Subjects:
Dentistry
DNA methylation
Gene amplification
Homeobox genes
Oral squamous cell carcinoma
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Summary:•Homeobox genes HOXA9, HOXB13 and IRX4 are amplified in oral squamous cell carcinoma.•HOXD10 was hypermethyladed in oral squamous cell carcinoma.•Genetic alterations may contribute to deregulated expression of homeobox genes. Investigate the DNA copy number and the methylation profile of the homeobox genes HOXA5, HOXA7, HOXA9, HOXB5, HOXB13, HOXC12, HOXC13, HOXD10, HOXD11, IRX4 and ZHX1, and correlate them with clinicopathological parameters and overall survival. DNA from OSCC samples and surgical margins were submitted to DNA amplification by qPCR and to DNA methylation analysis using a DNA Methylation PCR Array System. HOXA5, HOXB5 and HOXD10 were amplified in surgical margins while HOXA9, HOXB13 and IRX4 were amplified in OSCC. HOXD10 demonstrated hypermethylation in half of the tumor while ZHX1 did not show hypermethylation. No correlation of DNA copy number or methylation with clinicopathological parameters or survival was observed. HOXA9, HOXB13 and IRX4 genes appears to be regulated by amplification and HOXD10 by methylation in OSCC. Further studies are needed to determine the role of these events in OSCC development.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2021.105195