Halomonas azerica sp. nov., Isolated from Urmia Lake in Iran

• Published in: Current microbiology 2021-08, Vol.78 (8), p.3299-3306
• Main Authors: Wenting, Ruan, Montazersaheb, Soheila, Khan, Shehzad Abid, Kim, Hyung Min, Tarhriz, Vahideh, Hejazi, Mohammad Amin, Hejazi, Mohammad Saeid, Jeon, Che Ok
• Format: Article
• Language: English
• Subjects: Bacteria; Biomedical and Life Sciences; Biotechnology; Deoxyribonucleic acid; DNA; Fatty acids; Genes; Genomes; Halomonas; Hybridization; Lakes; Life Sciences; Lipids; Microbiology; New species; Nucleotides; pH effects; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phylogenetics; Phylogeny; Physiology; Quinones; rRNA 16S; Sodium chloride
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-021-02482-0

A novel moderately halophilic, Gram-staining negative and facultative aerobic bacterium, designated as TBZ9 T , was isolated from water of Urmia Lake in Azerbaijan region of Iran. The cells were found to be rod-shaped and motile, growing in the form of creamy, convex and shiny colonies. The strain could grow in the presence of NaCl at concentrations 1–17% (w/v) (optimum, 3%), temperatures 10–40 °C (optimum, 30 °C) and pH 6.0–11.0 (optimum, pH 7.0) on marine agar. Strain TBZ9 T 16S rRNA gene sequence was related to the genus Halomonas showing highest similarities to Halomonas arcis AJ282 T (98.4%), Halomonas songnenensis NEAU-ST10-39 T (98.0%) and Halomonas lutescens Q1U T (97.8%). In the phylogenetic trees, strain TBZ9 T formed a distinct branch closely related to a subclade inside the Halomonas genus. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain TBZ9 T and H. arcis AJ282 T (20.0%, 74.0%) and H. songnenensis NEAU-ST10-39 T (19.8%, 75.2%) indicated that TBZ9 T represents a distinct species. Evaluation of fatty acid contents determined C 10:0 , C 16:0 , C 12:0 3-OH, summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ) and summed feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ) as major cellular fatty acids. The major quinone of strain TBZ9 T was Q-9. Polar lipid patterns consisted of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified phospholipids (PL) and four unidentified polar lipids (L). The average DNA G + C content of strain TBZ9 T is 55.4 mol%. Results from phenotypic, chemotaxonomic and molecular analysis suggest that the strain TBZ9 T represents a novel species within the genus Halomonas for which the name Halomonas azerica sp. nov. is proposed. The type strain is TBZ9 T (= KACC 21783 T  = LMG 25775 T ).