A Dual Functional Self-Enhanced Electrochemiluminescent Nanohybrid for Label-Free MicroRNA Detection

The development of electrochemiluminescent (ECL) emitters with both intense ECL and excellent film-forming properties is highly desirable for biosensing applications. Herein, a facile one-pot preparation strategy was proposed for the synthesis of a self-enhanced ECL emitter by co-doping Ru­(bpy)3 2+...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-06, Vol.93 (25), p.8971-8977
Main Authors: Ning, Zhenqiang, Yang, Erli, Zheng, Yongjun, Chen, Mengyuan, Wu, Guoqiu, Zhang, Yuanjian, Shen, Yanfei
Format: Article
Language:English
Subjects:
Analytical chemistry
Biosensors
Chemistry
Electrochemiluminescence
Emitters
MicroRNAs
miRNA
Silica
Silicon dioxide
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Summary:The development of electrochemiluminescent (ECL) emitters with both intense ECL and excellent film-forming properties is highly desirable for biosensing applications. Herein, a facile one-pot preparation strategy was proposed for the synthesis of a self-enhanced ECL emitter by co-doping Ru­(bpy)3 2+ and (diethylaminomethyl)­triethoxysilane (DEAMTES) into an in situ-produced silica nanohybrid (DEAMTES@RuSiO2). DEAMTES@RuSiO2 not only possessed improved ECL properties but also exhibited outstanding film-forming ability, which are both critical for the construction of ECL biosensors. By coupling branched catalytic hairpin assembly with efficient signal amplification peculiarity, a label-free ECL biosensor was further constructed for the convenient and highly sensitive detection of miRNA-21. The as-fabricated ECL biosensor displayed a detection limit of 8.19 fM, much lower than those in previous reports for miRNA-21 and showed superior reliability for detecting miRNA-21-spiked human serum sample, demonstrating its potential for applications in miRNA-associated fundamental research and clinical diagnosis.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c01570