Antiproliferative labdane diterpenes from the rhizomes of Hedychium flavescens Carey ex Roscoe

• Published in: Chemical biology & drug design 2021-10, Vol.98 (4), p.501-506
• Main Authors: Subramanyan, Santhi, Deepika, Selvakumar, Ajith, Anjitha, Joseph Gracy, Anuja, Dan, Mathew, Maiti, Kaustabh Kumar, Varma, Ramavarma Luxmi, Radhakrishnan, Kokkuvayil Vasu
• Format: Article
• Language: English
• Subjects: A549 Cells; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; antiproliferative activity; Apoptosis - drug effects; Cell Proliferation - drug effects; coronarin E; Diterpenes - chemistry; Diterpenes - pharmacology; Doxorubicin - pharmacology; Doxorubicin - standards; Drug Screening Assays, Antitumor; Hedychium flavescens; Humans; labdane diterpenes; Phytochemicals - chemistry; Phytochemicals - pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Rhizome - chemistry; Zingiberaceae; Zingiberaceae - chemistry
• ISSN: 1747-0277; 1747-0285
• DOI: 10.1111/cbdd.13906

Antiproliferative activity was confirmed in the various extracts of rhizomes of Hedychium flavescens (Zingiberaceae). The phytochemical investigation of the rhizomes of Hedychium flavescens led to the isolation of four labdane diterpenes. Their structures were established as coronarin E (1), C‐14 epimers of isocoronarin D (2), C‐15 epimers of coronarin D methyl ether (3) and isocoronarin D (4). The structure of the compounds was identified based on spectroscopic analysis and on comparison with literature reports. All these compounds were assessed for their in vitro cytotoxicity against human lung adenocarcinoma (A549) cell line and showed significant cytotoxicity as reflected in IC50 value, that is, 0.52, 0.59, 0.68 and 1.22 μM compared with the control doxorubicin (IC50 0.92 μM). Moreover, all the compounds were nontoxic towards the normal lung fibroblast (WI‐38) cells. The chemo‐profiling and cytotoxicity study of Hedychium flavescens is reported for the first time. It is the first report on the phytochemical investigation and antiproliferative study of rhizomes of Hedychium flavesence Carey ex Roscoe. Four labdane diterpenes (1–4) were isolated, and their structures were established by spectroscopic analyses. All the compounds exhibited selective cytotoxicity against human lung adenocarcinoma (A549) cell line with an IC50 ranging from 0.52 to 1.22 µM.