Devosia sediminis sp. nov., isolated from subterranean sediment

• Published in: Archives of microbiology 2021-09, Vol.203 (7), p.4517-4523
• Main Authors: Ma, Fu, Zi, Zhi-Da, Li, Wei, Wang, Zhao-Xuan, Lu, Jun, Lv, Jie
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Biotechnology; Cardiolipin; Cell Biology; Deoxyribonucleic acid; Devosia; DNA; Ecology; Fatty acids; Glycolipids; Life Sciences; Lipids; Microbial Ecology; Microbiology; New species; Novel Prokaryotic Taxa; Original Paper; pH effects; Phosphatidylglycerol; Phospholipids; Phylogenetics; Phylogeny; Quinones; rRNA 16S; Sediment samplers; Sodium chloride; Ubiquinone
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02448-7

A novel Gram-negative, cream-colored, rod-shaped, aerobic, non-motile bacterium, designated MSA67 T , was isolated from a subterranean sediment sample of the Mohe Basin in Northeast China. Strain MSA67 T was detected to grow at 4–40 °C (optimum 28–30 °C), pH 5.0–10.0 (optimum, pH 7.0) and in 0.0–8.0% ( w / v ) NaCl (optimum 2.0–3.0%). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain MSA67 T was a member of the genus Devosia , with the highest similarity with D. riboflavina IFO13584 T (98.0%) and D. chinhatensis IPL18 T (97.0%). The major cellular fatty acids are C 16:0 , C 18:1 ω 7 c 11-methyl and C 18:1 ω 6 c and/or C 18:1 ω 7 c . The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, glycolipids and three unidentified phospholipids. The major respiratory quinone is ubiquinone 10 (Q-10). The genomic size of strain MSA67 T is 4.1 MB and DNA G + C content is 63.6%. Based on genotypic, phenotypic and phylogenetic results, strain MSA67 T is concluded to represent a novel species of the genus Devosia , for which the name Devosia sediminis sp. nov. is proposed. The type strain is MSA67 T (= CGMCC 1.18467 T  = KCTC 82192 T ).