High-throughput subzero-temperature assisted homogenous liquid-liquid extraction for the fast sample preparation of multiple phenolic compounds in propolis

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2021-08, Vol.1179, p.122823-122823, Article 122823
• Main Authors: Yu, Fengjie, Zhang, Jianing, Tao, Yunmin, Du, Chunping, Yang, Wenchao, Chen, Wenbin, Tu, Xijuan
• Format: Article
• Language: English
• Subjects: Cold Temperature; Green analytical chemistry; High-throughput; High-Throughput Screening Assays - methods; Homogenous liquid-liquid extraction; Limit of Detection; Linear Models; Liquid-Liquid Extraction - methods; Phenols - analysis; Phenols - isolation & purification; Propolis - chemistry; Reproducibility of Results; Sample preparation; Subzero temperature
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2021.122823

•A high-throughput (HT) homogenous liquid-liquid extraction method was developed.•The proposed HT method was extremely rapid and eco-friendly.•HT samples array was performed and accomplished simultaneously in 5 min.•HT extraction of phenolic targets from propolis showed good analytical performance. In the present study, a high-throughput homogenous liquid-liquid extraction method was developed for fast sample preparation of multiple phenolic compounds in propolis. This method was proposed based on cooling samples array in subzero temperature to induce phase separation of ACN-H2O extractant. Due to the high-throughput ability, optimization of extraction parameters was rapidly achieved by using a 5 × 4 × 3 samples array. In addition, multiple arrays were investigated for evaluating the analytical performance of the high-throughput method, which indicated that limits of detection and quantification were ranged from 0.04 to 0.35 µg/mL and 0.12 to 1.05 µg/mL, respectively. Recoveries and precisions in inter-day high-throughput studies were in the range of 90.55–105.50% and 2.58–4.30%, respectively. Comparing with the conventional liquid extraction method, this ecofriendly high-throughput method presented remarkable advantages in reducing sample and chemical consumption, as well as saving labor and time cost. The proposed method might provide a valuable strategy for the design of high-throughput extraction procedures.