Anchoring alien chromosome segment substitutions bearing gene(s) for resistance to mustard aphid in Brassica juncea-B. fruticulosa introgression lines and their possible disruption through gamma irradiation

• Published in: Theoretical and applied genetics 2021-10, Vol.134 (10), p.3209-3224
• Main Authors: Agrawal, Neha, Gupta, Mehak, Atri, Chhaya, Akhatar, Javed, Kumar, Sarwan, Heslop-Harrison, Pat (J. S.), Banga, Surinder S.
• Format: Article
• Language: English
• Subjects: Agriculture; Animals; Aphididae; Aphids - physiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Brassica juncea; Chromatin; Chromosome Mapping - methods; Chromosome translocations; Chromosomes; Chromosomes, Plant - genetics; Cultivars; Disease Resistance - genetics; Disease Resistance - immunology; Disease Resistance - radiation effects; DNA probes; Gamma Rays; Gene Expression Regulation, Plant; Genetic research; Genomes; Genomic in situ hybridization; Genomics; Life Sciences; Mustard Plant - genetics; Mustard Plant - growth & development; Mustard Plant - parasitology; Mustard Plant - radiation effects; Oligonucleotides; Original Article; Pest resistance; Plant Biochemistry; Plant breeding; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - parasitology; Plant Genetics and Genomics; Plant Proteins - genetics; Plant Proteins - metabolism; Seeds; Single nucleotide polymorphisms; Single-nucleotide polymorphism; γ Radiation
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-021-03886-z

Key message Heavy doses of gamma irradiation can reduce linkage drag by disrupting large sized alien translocations and promoting exchanges between crop and wild genomes. Resistance to mustard aphid ( Lipaphis erysimi ) infestation was significantly improved in Brassica juncea through B. juncea - B. fruticulosa introgression. However, linkage drag caused by introgressed chromatin fragments has so far prevented the deployment of this resistance source in commercial cultivars. We investigated the patterns of donor chromatin segment substitutions in the introgression lines (ILs) through genomic in situ hybridization (GISH) coupled with B. juncea chromosome-specific oligonucleotide probes. These allowed identification of large chromosome translocations from B. fruticulosa in the terminal regions of chromosomes A05, B02, B03 and B04 in three founder ILs (AD-64, 101 and 104). Only AD-101 carried an additional translocation at the sub-terminal to intercalary position in both homologues of chromosome A01. We validated these translocations with a reciprocal blast hit analysis using shotgun sequencing of three ILs and species-specific contigs/scaffolds (kb sized) from a de novo assembly of B. fruticulosa . Alien segment substitution on chromosome A05 could not be validated. Current studies also endeavoured to break linkage drag by exposing seeds to a heavy dose (200kR) of gamma radiation. Reduction in the size of introgressed chromatin fragments was observed in many M 3 plants. There was a complete loss of the alien chromosome fragment in one instance. A few M 3 plants with novel patterns of chromosome segment substitutions displayed improved agronomic performance coupled with resistance to mustard aphid. SNPs in such genomic spaces should aid the development of markers to track introgressed DNA and allow application in plant breeding.