Cell–surface interactions on gold‐coated polydimethylsiloxane nanocomposite structures: Localized laser heating on cell viability

• Published in: Journal of biomedical materials research. Part A 2021-12, Vol.109 (12), p.2611-2624
• Main Authors: Danyuo, Yiporo, Obayemi, John David, Salifu, Ali Azeko, Oyewole, Oluwaseun Kehinde, Azeko, ‪Salifu Tahiru, Ani, Chukwuemeka Joseph, Dozie‐Nwachukwu, Stella, Yirijor, John, Abade‐Abugre, Miriam, Odusanya, Olushola Segun, McBagonluri, Fred, Soboyejo, Winston Oluwole
• Format: Article
• Language: English
• Subjects: Adhesion; Biocompatibility; Biomedical materials; Breast cancer; Cell Adhesion; Cell Line, Tumor; Cell proliferation; Cell Proliferation - drug effects; Cell surface; Cell Survival - drug effects; Cell viability; cell–surface interactions; Coatings; Cytotoxicity; Dimethylpolysiloxanes - chemistry; Female; Ferrosoferric Oxide; Fluorescence; Fluorescence microscopy; Gold; Gold - pharmacology; Gold coatings; Hot Temperature; Humans; implantable biomedical devices; Laser beam heating; Lasers; laser–materials interactions; Magnetite; Metal Nanoparticles - chemistry; Nanocomposites; Nanocomposites - chemistry; PDMS nanocomposites; Photothermal conversion; Polydimethylsiloxane; Prostheses and Implants; Substrates; Surface Properties; Thin films; Titanium; Titanium - chemistry; Toxicity; Vapor deposition
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.37254

This article presents the results of cell–surface interactions on polydimethylsiloxane (PDMS)‐based substrates coated with nanoscale gold (Au) thin films. The surfaces of PDMS and PDMS–magnetite (MNP)‐based substrates were treated with UV‐ozone, prior to thermal vapor deposition (sputter‐coated) of thin films of titanium (Ti) onto the substrates to improve the adhesion of Au coatings. The thin layer of Ti was thermally evaporated to improve interfacial adhesion, which was enhanced by a 40‐nm thick film microwrinkled/buckled wavy layer of Au, that was coated to enhance cell–surface interactions and protein absorption. Cell–surface interactions were studied on the hybrid surfaces using a combination of optical and fluorescence microscopy. Consequently, cell proliferation and surface cytotoxicity (of the sputter‐coated PDMS surfaces) were elucidated by characterizing the metabolic activity in the presence of breast cancer and normal breast cells. The photothermal conversion efficiency associated with laser–materials interactions with the PDMS/PDMS–magnetite‐based composites was shown to have an optimum efficiency of ~31.8%. The implications of the results are discussed for potential applications of PDMS nanocomposites in implantable biomedical devices.