The effect of aerobic exercise on the expression of mir-126 and related target genes in the endothelial tissue of the cardiac muscle of diabetic rats

The aim of this study was to investigate the effect of 8 weeks of aerobic exercise on the expression of mir-126 and some angiogenesis factors in the endothelial tissue of the cardiac muscle of type 2 diabetic rats. Sixteen male Wistar rats were divided into two groups: diabetic control and diabetic...

Full description

Saved in:
Bibliographic Details
Published in:Microvascular research 2021-11, Vol.138, p.104212-104212, Article 104212
Main Authors: Dastah, Samaneh, Tofighi, Asghar, Bonab, Solmaz Babaei
Format: Article
Language:English
Subjects:
Aerobic exercise
Animals
Blood Glucose - metabolism
Diabetes
Diabetes Mellitus, Experimental - genetics
Diabetes Mellitus, Experimental - metabolism
Diabetes Mellitus, Experimental - physiopathology
Diabetes Mellitus, Type 2 - genetics
Diabetes Mellitus, Type 2 - metabolism
Diabetes Mellitus, Type 2 - physiopathology
Endothelial Cells - metabolism
Gene Expression Regulation
Insulin Resistance
Male
MicroRNAs - genetics
MicroRNAs - metabolism
Mir-126
Myocardium - metabolism
Phosphatidylinositol 3-Kinase - genetics
Phosphatidylinositol 3-Kinase - metabolism
Physical Conditioning, Animal
Physical Exertion
PI3K
Proto-Oncogene Proteins c-raf - metabolism
raf1
Rats
Rats, Wistar
Vascular Endothelial Growth Factor A - metabolism
VEGF
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this study was to investigate the effect of 8 weeks of aerobic exercise on the expression of mir-126 and some angiogenesis factors in the endothelial tissue of the cardiac muscle of type 2 diabetic rats. Sixteen male Wistar rats were divided into two groups: diabetic control and diabetic training. Nicotinamide and streptozotocin injections were used to induce type 2 diabetes. After familiarization, the training group participated in an 8-week exercise protocol on a treadmill with an intensity of 25 m per minute, a slope of 5% and 30 min per session. RT-PCR was used to evaluate the expression of mir-126 and PI3K genes. Expression of raf1, VEGF, blood glucose and insulin was determined by ELISA and insulin resistance was assessed by HOMA-IR homeostasis model. Immunohistochemistry was used to measure the capillary density of the cardiac muscle. Data were analyzed by t-test for independent groups with a significance level of p 
ISSN:0026-2862
1095-9319
DOI:10.1016/j.mvr.2021.104212