Vitellogenin regulates estrogen-related receptor expression by crosstalk with the JH and IIS-TOR signaling pathway in Polyrhachis vicina Roger (Hymenoptera, Formicidae)

• Published in: General and comparative endocrinology 2021-09, Vol.310, p.113836-113836, Article 113836
• Main Authors: Zhang, Juan-Juan, Xi, Geng-Si, Zhao, Jing
• Format: Article
• Language: English
• Subjects: Animals; Ants - genetics; Estrogen-related receptor; Estrogens; Female; IIS/TOR pathway; Insect Proteins - genetics; Insect Proteins - metabolism; JH pathway; Juvenile Hormones - metabolism; Male; Polyrhachis vicina Roger; Receptors, Estrogen - metabolism; RNA Interference; Signal Transduction; Vitellogenin; Vitellogenins - genetics; Vitellogenins - metabolism
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2021.113836

•PvVg gene exhibited a developmental stage-, tissue- and sex-specific expression.•JH regulates PvVg synthesis through IIS/TOR pathway to influence ERR expression.•Interaction of PvERR with PvVg depends on developmental stage and caste difference. The Estrogen-related receptor (ERR) can regulate the growth and development, metabolism, reproduction, and other physiological activities of insects, but its specific mechanism of action is still unclear. The aim of this study was to explore the relationship between expression of ERR and Vitellogenins (Vg) and the juvenile hormone (JH) and insulin/insulin-like growth factor/target of rapamycin (IIS/TOR) signaling pathways in Polyrhachis vicina Roger. P. vicina was used as the experimental model to clone the PvVg gene, perform double-stranded RNA synthesis and delivery and observe the effects of pharmacological treatments. The full-length PvVg cDNA product is 5586 bp. Higher PvVg mRNA expression was seen in the pupa and adults, and varying levels were seen in the different body parts of three different castes. RNA interference of PvVg expression led to disturbed development, an abnormal phenotype, and high mortality. PvVg RNAi also led to a reduction in mRNA levels of PvERR, ultraspiracle (PvUSP), forkhead box protein O (PvFOXO) and PvTOR genes in fourth instar larval, but a significant increase was seen in pupa and females. No significant change was seen in workers and males. After PvVg knockdown, application of exogenous JHIII reduced the expression of these genes in pupa and females, increased expression in workers, and decreased PvUSP mRNA expression in males. Both protein and mRNA expression levels of PvFOXO were affected by PvVg RNAi. PvERR RNAi increased PvVg expression in pupa and females and Kruppel-homolog 1 (PvKr-h1) and PvFOXO expression in males. The results of this study suggest that there is an interaction between PvERR and PvVg, and that crosstalk with the JH and IIS/TOR signaling pathways can affect development and reproduction. This effect is caste and developmental stage specific. We also speculate that the FOXO/USP complex participates in JH regulation of PvVg in P. vicina.