Influence of light and flow on taxon composition and photosynthesis of marine phototrophic biofilm in photobioreactors

• Published in: Marine environmental research 2021-07, Vol.169, p.105395-105395, Article 105395
• Main Authors: Guzzon, A., Di Pippo, F., Bonavita, S., Congestri, R.
• Format: Article
• Language: English
• Subjects: Algae; Aquatic plants; Bacillariophyceae; Biofilms; Biofouling; Chlorophyta; Composition effects; Cyanobacteria; Diatoms; Electron microscopy; Flow rates; Flow velocity; Fluorescence; Irradiance; Marine microorganisms; Microscopy; Photobioreactor; Photobioreactors; Photosynthesis; Phototrophic community; Phototrophic marine biofilms; Pulse amplitude; Pulse amplitude modulated fluorescence; Scanning electron microscopy
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/j.marenvres.2021.105395

In this study, marine biofilms were cultured in a flow-lane, semi-continuous photobioreactor at different irradiances and flows to evaluate their combined effect on biofilms' phototrophic composition and photosynthetic activity. Taxon richness, evaluated by different microscopy techniques, including transmission and scanning electron microscopy, resulted to be heavily reduced from source communities to mature cultures. The strongest decrease was observed for diatoms, which were overcome by cyanobacteria and green algae over time. Photosynthetic performance was investigated by pulse amplitude modulated fluorescence. Irradiance was the main driver of data distribution of the photosynthetic parameters rel.ETRmax and Ik, while flow rate affected α and ΔF/Fm’. The combination of irradiance and flow rate affected ΔF/Fm’ reflecting the photosynthetic performance of the most relatively abundant taxa. Higher ΔF/Fm’ was attained when cyanobacteria and green algae were dominating, whilst lower ΔF/Fm’ when diatoms occurred in the initial phase of biofilm development. •Marine phototrophic biofilms were grown in a flow-lane photobioreactor.•Light and flow impacted culture's phototroph composition and photosynthesis.•Mature biofilms developed at the highest irradiances at the two flow conditions.•Diatoms were overcome by cyanobacteria and green algae over time.•Light was driving rel.ETRmax and Ik, data distribution while flow affected α and ΔF/Fm’.