Hepato and nephroprotective activity of methanol extract of Hygrophila spinosa and its antibacterial potential against multidrug resistant Pandoraea sputorum

• Published in: Environmental research 2021-10, Vol.201, p.111594-111594, Article 111594
• Main Authors: Narayanan, Mathiyazhagan, Gopi, A., Natarajan, Devarajan, Kandasamy, Sabariswaran, Saravanan, Mythili, El Askary, Ahmad, Elfasakhany, Ashraf, Pugazhendhi, Arivalagan
• Format: Article
• Language: English
• Subjects: Acanthaceae - chemistry; Anti-Bacterial Agents - pharmacology; Antioxidant; Bactericidal; Burkholderiaceae - drug effects; Drug Resistance, Multiple, Bacterial; HEK293 Cells; Hep G2 Cells; Hepatoprotective; Humans; Hygrophila spinosa; Kidney - drug effects; Liver - drug effects; Methanol; Nephroprotective; Pandoraea sputorum; Phytochemicals; Plant Extracts - pharmacology; Protective Agents - pharmacology
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2021.111594

This research was aimed to evaluate the phytochemical profile, bactericidal activity of Hygrophila spinosa against multidrug resistant Pandoraea sputorum and assess their antioxidant competence against various radicals and studied their hepatoprotective and nephroprotective activity on HepG2 and HEK 293 cell line. The results showed that the methanol extract has various phytochemical components with reasonable quantity. Fortunately, the multidrug-resistant P. sputorum was sensitive (22.8 ± 0.2 mm of the zone of inhibition) at 15 mg mL−1 concentration of methanol extract. The higher concentration of phenolic and other phytochemical components, showed significant antioxidant activity against ferric, DPPH, hydroxyl, and ABTS radicals, with IC50 values of 71.09, 64.333, 91.157, and 104.931 g mL−1, respectively. Surprisingly, the methanol extract possesses hepato and nephroprotective activity against CCl4 and cisplatin-induced cytotoxicity on HepG2 and HEK 293 cell lines, respectively. It maintains the cell viability as up to 90.48% and 90.35% of HepG2 and EK 293 cell line at the concentration of 20 μg mL−1. The FTIR analysis states that the methanol extract possesses a significant functional group responsible for these multi-potential activities. These results suggest that, the methanol extract of H. spinosa might contain the most significant bioactive components with outstanding medicinal properties. •Emerging multidrug resistant P. sputorum isolated from magnesite mine tailing.•First report on bactericidal activity of H. spinosa methanol extract on P. sputorum.•Methanol extract showed excellent antioxidant potential on multiple free radicals.•Methanol extract showed excellent hepatoprotectivity of HepG2 cell line.•Foremost report on nephroprotective potential of H. spinosa on HEK293 cell line.