Modulation of frequency and height of cytosolic calcium spikes by plasma membrane anion channels in guard cells

Cytosolic calcium ([Ca2+]cyt) elevation activates plasma membrane anion channels in guard cells, which is required for stomatal closure. However, involvement of the anion channels in the [Ca2+]cyt elevation remains unclear. We investigated the involvement using Arabidopsis thaliana anion channel mut...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2021-08, Vol.85 (9), p.2003-2010
Main Authors: Tahjib-Ul-Arif, Md, Munemasa, Shintaro, Nakamura, Toshiyuki, Nakamura, Yoshimasa, Murata, Yoshiyuki
Format: Article
Language:English
Subjects:
Anions
Arabidopsis - genetics
Arabidopsis - metabolism
Calcium - metabolism
Calcium Signaling
Cell Membrane - metabolism
Cytosol - metabolism
Gene Knockdown Techniques
Genes, Plant
Ion Channels - metabolism
Mutation
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Summary:Cytosolic calcium ([Ca2+]cyt) elevation activates plasma membrane anion channels in guard cells, which is required for stomatal closure. However, involvement of the anion channels in the [Ca2+]cyt elevation remains unclear. We investigated the involvement using Arabidopsis thaliana anion channel mutants, slac1-4 slah3-3 and slac1-4 almt12-1. Extracellular calcium induced stomatal closure in the wild-type plants but not in the anion channel mutant plants whereas extracellular calcium induced [Ca2+]cyt elevation both in the wild-type guard cells and in the mutant guard cells. The peak height and the number of the [Ca2+]cyt spike were lower and larger in the slac1-4 slah3-3 than in the wild type and the height and the number in the slac1-4 almt12-1 were much lower and much larger than in the wild type. These results suggest that the anion channels are involved in the regulation of [Ca2+]cyt elevation in guard cells.
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbab118