The morphine/heroin vaccine decreased the heroin-induced antinociceptive and reinforcing effects in three inbred strains mouse

• Published in: International immunopharmacology 2021-09, Vol.98, p.107887-107887, Article 107887
• Main Authors: Barbosa-Méndez, Susana, Matus-Ortega, Maura, Hernández-Miramontes, Ricardo, Salazar-Juárez, Alberto
• Format: Article
• Language: English
• Subjects: Active vaccination; Analgesics, Opioid; Animal models; Animals; Antibodies; Clinical trials; Disease Models, Animal; Enzyme-linked immunosorbent assay; Female; Heroin; Heroin - adverse effects; Heroin - immunology; Human populations; Humans; Immune response; Immune system; Immunization; Immunogenicity; Immunogenicity, Vaccine; Inbreeding; Locomotor activity; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred DBA; Morphine; Morphine - adverse effects; Morphine - immunology; M−TT vaccine; Nociception; Nociception, inbred mouse strain; Opioid-Related Disorders - immunology; Opioid-Related Disorders - therapy; Pain perception; Place preference; Population genetics; Populations; Reinforcement, Psychology; Solid phases; Vaccines; Vaccines - administration & dosage; Vaccines - immunology
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2021.107887

•M6-TT vaccine decrease the heroin-induced analgesia in three inbred mouse strain.•M6-TT vaccine decrease heroin-locomotor sensitization in three inbred mouse strain.•M6-TT vaccine decrease heroin-induced place-preference in three inbred mouse strain. Clinical trials have indicated that a vaccine must be immunogenic in genetically diverse human populations and that immunogenicity and protective efficacy in animal models are two key indices required for the approval of a new vaccine. Additionally, the immune response (immunogenicity) and immunoprotection are dependent on the mouse strain. Therefore, the objective of the present study was to determine the immune response (immunogenicity) and the protective efficacy (behavioral response) in three inbred mouse strains immunized with the M6TT vaccine. Female BALB/c, C57Bl/6, and DBA/2 inbred mice were immunized with the M6-TT vaccine. A solid-phase antibody-capture ELISA was used to monitor antibody titer responses after each booster dose in vaccinated animals. The study used tail-flick testing to evaluate the antinociceptive effects induced by heroin. Additionally, heroin-induced locomotor activity and place preference were evaluated. The M6-TT vaccine was able to generate a specific antibody titer in the three inbred mouse strains evaluated. The antibodies reduced the antinociceptive effect of different doses of heroin. In addition, they decreased the heroin-induced locomotor activity and place preference. These findings suggest that the M6-TT vaccine generates a powerful immunogenic response capable of reducing the antinociceptive and reinforcing effects of heroin in different inbred mouse strains, which supports its possible future use in clinical trials in genetically diverse human populations.