The inhibition of WIP1 phosphatase accelerates the depletion of primordial follicles

• Published in: Reproductive biomedicine online 2021-08, Vol.43 (2), p.161-171
• Main Authors: Zhou, Su, Xi, Yueyue, Chen, Yingying, Wu, Tong, Yan, Wei, Li, Milu, Wu, Meng, Luo, Aiyue, Shen, Wei, Xiang, Tao, Wang, Shixuan
• Format: Article
• Language: English
• Subjects: Aminopyridines - pharmacology; Animals; Animals, Newborn; Apoptosis; Cell Count; Cells, Cultured; Dipeptides - pharmacology; Enzyme Inhibitors - pharmacology; Female; Male; Mice; Mice, Inbred C57BL; Ovarian Follicle - cytology; Ovarian Follicle - drug effects; Ovarian Follicle - physiology; Ovary - cytology; Ovary - drug effects; Pregnancy; Primordial follicle activation; Primordial follicle atresia; Protein Phosphatase 2C - antagonists & inhibitors; WIP1
• ISSN: 1472-6483; 1472-6491
• DOI: 10.1016/j.rbmo.2021.05.007

What role does wild-type p53-induced phosphatase 1 (WIP1) play in the regulation of primordial follicle development? WIP1 expression was detected in the ovaries of mice of different ages by western blotting and immunohistochemical staining. Three-day-old neonatal mouse ovaries were cultured in vitro with or without the WIP1 inhibitor GSK2830371 (10 μM) for 4 days. Ovarian morphology, follicle growth and follicle classification were analysed and the PI3K–AKT–mTOR signal pathway and the WIP1–p53-related mitochondrial apoptosis pathway evaluated. WIP1 expression was downregulated with age. Primordial follicles were significantly decreased in the GSK2830371-treated group, without a significant increase in growing follicles. The ratio of growing follicles to primordial follicles was not significantly different between the control and GSK2830371 groups, and no significant variation was observed in the PI3K–AKT–mTOR signal pathway. The inhibition of WIP1 phosphatase accelerated primordial follicle atresia by activating the p53–BAX–caspase-3 pathway. These findings reveal that WIP1 participates in regulating primordial follicle development and that inhibiting WIP1 phosphatase leads to massive primordial follicle loss via interaction with the p53–BAX–caspase-3 pathway. This might also provide valuable information for understanding decreased ovarian reserve during ovarian ageing.