Prenatal hypoxia affects scaling of blood pressure and arterial wall mechanics in the common snapping turtle, Chelydra serpentina

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2021-10, Vol.260, p.111023-111023, Article 111023
• Main Authors: Filogonio, Renato, Dubansky, Benjamin D., Dubansky, Brooke H., Leite, Cléo A.C., Crossley, Dane A.
• Format: Article
• Language: English
• Subjects: Animals; Arterial blood pressure; Blood Pressure; Body Mass Index; Collagen content; Developmental programming; Embryo, Nonmammalian - physiology; Female; Heart; Heart Rate - physiology; Hypoxia - physiopathology; Law of Laplace; Lung; Oxygen; Phenotypic plasticity; Pulmonary Artery - physiology; Pulmonary Artery - physiopathology; Turtles - embryology; Turtles - physiology
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpa.2021.111023

In reptiles, exposure to hypoxia during embryonic development affects several cardiovascular parameters. These modifications may impose different mechanical stress to the arterial system, and we speculated that the arterial wall of major outflow vessels would be modified accordingly. Since non-crocodilian reptiles possess a partially divided ventricle, ensuing similar systemic and pulmonary systolic pressures, we investigated how morphological and mechanical properties of segments from the left aortic arch (LAo) and the proximal and distal segments of the left pulmonary artery (LPAp and LPAd, respectively) change as body mass (Mb) increases. Eggs from common snapping turtles, Chelydra serpentina, were incubated under normoxia (21% O2; N21) or hypoxia (10% O2; H10), hatched and maintained in normoxia thereafter. Turtles (0.11–6.85 kg) were cannulated to measure arterial pressures, and an injection of adrenaline was used to increase pressures. Portions of the LAo, LPAp and LPAd were fixed under physiological hydrostatic pressures for histology and mechanical assessment. Arterial pressures increased with Mb for N21 but not for H10. Although mechanical and functional characteristics from the LPAp and LPAd were similar between N21 and H10, wall thickness from LAo did not change with Mb in the H10 group, thus wall stress increased in larger turtles. This indicates that larger H10 turtles probably experience an elevated probability of arterial wall rupture without concomitant changes in the cardiovascular system to prevent it. Finally, collagen content of the LPAp and LAo was smaller than in LPAd, suggesting a more distensible arterial wall could attenuate higher pressures from larger turtles. [Display omitted] •Arterial wall thickness did not increase with body mass in hypoxic reared turtles.•Wall stress increased with body mass in turtles incubated under hypoxia.•Proximal and distal pulmonary artery segments differed in morphology and mechanics.•Pulmonary wall tension in turtles was higher compared to alligators similar in size.