Analysis of genomic characteristics and their influence on metabolism in Aspergillus luchuensis albino mutants using genome sequencing

• Published in: Fungal genetics and biology 2021-10, Vol.155, p.103601-103601, Article 103601
• Main Authors: Yamamoto, Nozomi, Watarai, Naoki, Koyano, Hitoshi, Sawada, Kazunori, Toyoda, Atsushi, Kurokawa, Ken, Yamada, Takuji
• Format: Article
• Language: English
• Subjects: Albino mutant; Aspergillus - genetics; Aspergillus kawachii; Aspergillus luchuensis; Comparative genomics; Culture assay; Genomics; Industrial strain; Mutation; Phylogeny
• ISSN: 1087-1845; 1096-0937
• DOI: 10.1016/j.fgb.2021.103601

•Aspergillus luchuensis albino strains have other mutations than pigment deficiency.•Passaged albino strains are genetically distinct from A. luchuensis and A. kawachii.•Albino strains with isocitrate lyase mutation showed no growth with acetate.•Passage may influence the mutation of genes for stress responses in albino strains. Black Aspergillus luchuensis and its white albino mutant are essential fungi for making alcoholic beverages in Japan. A large number of industrial strains have been created using novel isolation or gene/genome mutation techniques. Such mutations influence metabolic and phenotypic characteristics in industrial strains, but few comparative studies of inter-strain mutation have been conducted. We carried out comparative genome analyses of 8 industrial strains of A. luchuensis and A. kawachii IFO 4308, the latter being the first albino strain to be isolated. Phylogenetic analysis based on 8938 concatenated genes exposed the diversity of black koji strains and uniformity among albino industrial strains, suggesting that passaged industrial albino strains have more genetic mutations compared with strain IFO 4308 and black koji strains. Comparative analysis showed that the albino strains had mutations in genes not only for conidial pigmentation but also in those that encode N-terminal acetyltransferase A and annexin XIV-like protein. The results also suggest that some mutations may have emerged through subculturing of albino strains. For example, mutations in the genes for isocitrate lyase and sugar transporters were observed only in industrial albino strains. This implies that selective pressure for increasing enzyme activity or secondary metabolites may have influenced the mutation of genes associated with environmental stress responses in A. luchuensis albino strains. Our study clarifies hitherto unknown genetic and metabolic characteristics of A. luchuensis industrial strains and provides potential applications for comparative genome analysis for breeding koji strains.