Functional assistance for stress distribution in cell culture membrane under periodically stretching

Dynamic cell cultures simulate the in vivo cell environment for a regular loading system with curtain strains. However, it is difficult to obtain strains that are suitable for cells without conducting multiple trials. This study develops a device that increases the strain gradient by changing the te...

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Bibliographic Details
Published in:Journal of biomechanics 2021-08, Vol.125, p.110564-110564, Article 110564
Main Authors: Dai, Zhi-Xuan, Shih, Po-Jen, Yen, Jia-Yush, Wang, I-Jong
Format: Article
Language:English
Subjects:
Cell adhesion
Cell culture
Cell proliferation
Cornea
Cyclic stretching
Experiments
Fibroblasts
Finite element analysis
Finite element analysis (FEA)
Human keratinocytes
Keratinocytes
Shear strain
Shear stress
Smooth muscle
Stem cells
Stress concentration
Stress distribution
Stretching
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Summary:Dynamic cell cultures simulate the in vivo cell environment for a regular loading system with curtain strains. However, it is difficult to obtain strains that are suitable for cells without conducting multiple trials. This study develops a device that increases the strain gradient by changing the tensile section, in order to determine the effect of various cyclic strains on cultured human keratinocytes (HK) cells. This device is used to determine the effect of 3% and 5% cyclic strain and shear strain on cell proliferation and arrangement at 1 Hz. The results show that compared with static and 3% strain, a 5% cyclic strain better inhibits the proliferation of HK cells. Compared to the initial cell attachment when there is no specific directionality, the cells are aligned in the vertical stretching direction after cyclic stretching. This equipment increases the efficiency of the experiment and more intuitively maps the cell behavior and shape to the strain field and the response to the shear strain.
ISSN:0021-9290
1873-2380
DOI:10.1016/j.jbiomech.2021.110564