Effect of processing methods on the cytotoxicity of methyl methacrylate-based ocular prostheses: An in vitro study

The study evaluated the influence of cycles and methods of an ocular prosthesis resin on cytotoxicity toward human conjunctival cells. Resins were polymerized by water bath (WB, 74 °C or 100 °C for 30 min to 9 h), microwave (MW, 1200 W, 3 to 14 min and 30 s at 0 to 720 W), or autopolymerization (AP,...

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Bibliographic Details
Published in:Toxicology in vitro 2021-10, Vol.76, p.105211-105211, Article 105211
Main Authors: da Silva, Emily Vivianne Freitas, Goiato, Marcelo Coelho, Bitencourt, Sandro Basso, Finer, Yoav, Brito, Victor Gustavo Balera, Takamiya, Aline Satie, de Oliveira, Sandra Helena Penha, dos Santos, Daniela Micheline
Format: Article
Language:English
Subjects:
Caspase 9 - genetics
Cell Line
Cell Proliferation
Cellular immune response
Conjunctiva - cytology
Cytokines - genetics
Cytokines - metabolism
Cytotoxicity
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Eye, Artificial
Humans
Materials Testing
Methyl methacrylate
Methylmethacrylate - chemistry
Methylmethacrylate - toxicity
Microwaves
Ocular prosthesis
Polymerization
Water - chemistry
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Summary:The study evaluated the influence of cycles and methods of an ocular prosthesis resin on cytotoxicity toward human conjunctival cells. Resins were polymerized by water bath (WB, 74 °C or 100 °C for 30 min to 9 h), microwave (MW, 1200 W, 3 to 14 min and 30 s at 0 to 720 W), or autopolymerization (AP, room temperature for 20 min ± 60 °C for 30 min). Degree of conversion (DC), cytotoxicity, level of inflammatory mediators, gene expression of different markers, and apoptosis were evaluated. Data were submitted to ANOVA and Tukey test (p 277 pg/mL). Short cycle medium power MW had higher IL-23 release (534.2 pg/mL). MW (long and short cycles) and AP polymerizations have triggered a more intense inflammatory response. Among methods recommended by the manufacturer, WB showed high DC and less cytotoxicity. •Different cycles and methods of processing ocular prosthesis resin were analyzed.•The material's cytotoxic activity toward human conjunctival cells was assessed.•The toxicity was analyzed in a cell line specific for human conjunctiva.•Some groups increased the production of inflammatory mediators.•Some groups showed lower degree of conversion.
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2021.105211