Concentrations of tissue inhibitor of matrix metalloproteinase‐1 and hyaluronic acid in canine amniotic membranes cryopreserved for different time points and its effects in dogs with complicated corneal ulcers

• Published in: Veterinary ophthalmology 2022-01, Vol.25 (1), p.62-72
• Main Authors: Dower, Nathalie Moro Bassil, Ribeiro, Alexandre Pinto, Gomes, Lianna Ghisi, Cássia Martini, Andresa, Taques, Isis Indaiara Gonçalves Granjeiro, Almeida, Sayanne Luns Hatum, Silva, Mayara Inácio Vincenzi, Aguiar, Daniel Moura
• Format: Article
• Language: English
• Subjects: Amnion - transplantation; Animals; canine amnion; corneal perforation; Corneal Ulcer - veterinary; descemetocele; Dog Diseases - surgery; Dogs; Female; graft age; Hyaluronic Acid; Matrix Metalloproteinase 1; Pregnancy; Tissue Inhibitor of Metalloproteinase-1; total protein
• ISSN: 1463-5216; 1463-5224
• DOI: 10.1111/vop.12916

Objectives To determine the concentrations of total protein (TP), tissue inhibitor of matrix metalloproteinase‐1 (TIMP‐1) and hyaluronic acid (HA) in amniotic membranes (AMs) harvested from placentas of bitches of different ages and cryopreserved for different time points. The outcomes of complicated corneal defects of dogs repaired with AMs stored for the same time points were also evaluated. Procedures Ten cryopreserved canine AMs were stored for short term (2–50 days), middle term (92–210 days), or long term (256–357 days). TP was quantified by Bradford’s test, whereas TIMP‐1 and HA were quantified by ELISA. Twenty‐one dogs that had an AM transplantation to restore deep or perforating corneal wounds were selected. Results TIMP‐1 levels were lower in AMs cryopreserved for middle term (p = .02) and long term (p = .0009), when compared to AMs stored for short term. TP (p = .39) and HA (p = .18) concentrations in AMs did not differ among the storage time. TIMP‐1 concentration in AMs correlated with storage time (R = −.65, p