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Concentrations of tissue inhibitor of matrix metalloproteinase‐1 and hyaluronic acid in canine amniotic membranes cryopreserved for different time points and its effects in dogs with complicated corneal ulcers
Objectives To determine the concentrations of total protein (TP), tissue inhibitor of matrix metalloproteinase‐1 (TIMP‐1) and hyaluronic acid (HA) in amniotic membranes (AMs) harvested from placentas of bitches of different ages and cryopreserved for different time points. The outcomes of complicate...
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| Published in: | Veterinary ophthalmology 2022-01, Vol.25 (1), p.62-72 |
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| container_title | Veterinary ophthalmology |
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| creator | Dower, Nathalie Moro Bassil Ribeiro, Alexandre Pinto Gomes, Lianna Ghisi Cássia Martini, Andresa Taques, Isis Indaiara Gonçalves Granjeiro Almeida, Sayanne Luns Hatum Silva, Mayara Inácio Vincenzi Aguiar, Daniel Moura |
| description | Objectives
To determine the concentrations of total protein (TP), tissue inhibitor of matrix metalloproteinase‐1 (TIMP‐1) and hyaluronic acid (HA) in amniotic membranes (AMs) harvested from placentas of bitches of different ages and cryopreserved for different time points. The outcomes of complicated corneal defects of dogs repaired with AMs stored for the same time points were also evaluated.
Procedures
Ten cryopreserved canine AMs were stored for short term (2–50 days), middle term (92–210 days), or long term (256–357 days). TP was quantified by Bradford’s test, whereas TIMP‐1 and HA were quantified by ELISA. Twenty‐one dogs that had an AM transplantation to restore deep or perforating corneal wounds were selected.
Results
TIMP‐1 levels were lower in AMs cryopreserved for middle term (p = .02) and long term (p = .0009), when compared to AMs stored for short term. TP (p = .39) and HA (p = .18) concentrations in AMs did not differ among the storage time. TIMP‐1 concentration in AMs correlated with storage time (R = −.65, p |
| doi_str_mv | 10.1111/vop.12916 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2550268362</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2550268362</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3256-6dba4e55c43cb415cf4240ef75b82d044e6d3a37f31206f4bfb25cbfbaffc1a43</originalsourceid><addsrcrecordid>eNp1kUtuFDEQhlsIREJgwQWQl7CYxO8JSzQKDylSWADbltsuM4X8aGx3wuw4AnfjBpwETyZkhxcuq_zXV3b9w_Cc0VPW19l1nk8Zf830g-GYSS1WinP58P7M9NHwpNZvlFKh6PrxcCQkl1RpcTz83uRkIbViGuZUSfakYa0LEExbnLDlss9F0wr-IBGaCSHPJTfAZCr8-fmLEZMc2e5MWEpOaImx6Ho1sSZhAmJiwtx6PkKciklQiS27zoAK5Roc8b2FQ--h9Hf07hHInDG1egvGHqFf2h471OWvldxg2xKb4xzQmtYRNpcEJpAlWCj16fDIm1Dh2V08GT6_vfi0eb-6vHr3YfPmcmUFV3ql3WQkKGWlsJNkyvr9VMCv1XTOHZUStBNGrL1gnGovJz9xZftuvLfMSHEyvDxw-zy-L1DbGLFaCKF_Mi915EpRrs-F5l366iC1JddawI9zwWjKbmR03Hs4dg_HWw-79sUddpkiuHvlP9O64OwguMEAu_-Txi9XHw_IvxY9roQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2550268362</pqid></control><display><type>article</type><title>Concentrations of tissue inhibitor of matrix metalloproteinase‐1 and hyaluronic acid in canine amniotic membranes cryopreserved for different time points and its effects in dogs with complicated corneal ulcers</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Dower, Nathalie Moro Bassil ; Ribeiro, Alexandre Pinto ; Gomes, Lianna Ghisi ; Cássia Martini, Andresa ; Taques, Isis Indaiara Gonçalves Granjeiro ; Almeida, Sayanne Luns Hatum ; Silva, Mayara Inácio Vincenzi ; Aguiar, Daniel Moura</creator><creatorcontrib>Dower, Nathalie Moro Bassil ; Ribeiro, Alexandre Pinto ; Gomes, Lianna Ghisi ; Cássia Martini, Andresa ; Taques, Isis Indaiara Gonçalves Granjeiro ; Almeida, Sayanne Luns Hatum ; Silva, Mayara Inácio Vincenzi ; Aguiar, Daniel Moura</creatorcontrib><description>Objectives
To determine the concentrations of total protein (TP), tissue inhibitor of matrix metalloproteinase‐1 (TIMP‐1) and hyaluronic acid (HA) in amniotic membranes (AMs) harvested from placentas of bitches of different ages and cryopreserved for different time points. The outcomes of complicated corneal defects of dogs repaired with AMs stored for the same time points were also evaluated.
Procedures
Ten cryopreserved canine AMs were stored for short term (2–50 days), middle term (92–210 days), or long term (256–357 days). TP was quantified by Bradford’s test, whereas TIMP‐1 and HA were quantified by ELISA. Twenty‐one dogs that had an AM transplantation to restore deep or perforating corneal wounds were selected.
Results
TIMP‐1 levels were lower in AMs cryopreserved for middle term (p = .02) and long term (p = .0009), when compared to AMs stored for short term. TP (p = .39) and HA (p = .18) concentrations in AMs did not differ among the storage time. TIMP‐1 concentration in AMs correlated with storage time (R = −.65, p < .0001), while TP (R = −.33, p = .07) and HA concentrations did not (R = −.15, p = .41). The age of donors did not correlate with the components evaluated in the AMs. Corneal defects repaired with AMs stored for short term healed sooner than the ones repaired with AMs stored for middle (p < .01) and long term (p = .02). Additionally, TIMP‐1 levels in AMs correlated negatively with the epithelization time (R = −.62, p = .002). Graft opacity was severe in 55% of cases. However, the HA levels in AMs correlated negatively with the opacification score (R = −.47, p = .03). Vision was observed in more patients who presented deep ulcers and descemetoceles, than in the ones with perforations (p = .004).
Conclusions
TIMP‐1 concentration in canine AMs significantly decreased over a year storage time, while TP and HA concentrations did not change during the same period. The age of donors did not correlate with the components evaluated in the AMs. Complicated corneal defects repaired with AMs cryopreserved for short term healed sooner and tended to be less opaque; however, satisfactory to optimal outcomes were achieved even in the eyes repaired with AMs stored for up to a year.</description><identifier>ISSN: 1463-5216</identifier><identifier>EISSN: 1463-5224</identifier><identifier>DOI: 10.1111/vop.12916</identifier><identifier>PMID: 34240563</identifier><language>eng</language><publisher>England</publisher><subject>Amnion - transplantation ; Animals ; canine amnion ; corneal perforation ; Corneal Ulcer - veterinary ; descemetocele ; Dog Diseases - surgery ; Dogs ; Female ; graft age ; Hyaluronic Acid ; Matrix Metalloproteinase 1 ; Pregnancy ; Tissue Inhibitor of Metalloproteinase-1 ; total protein</subject><ispartof>Veterinary ophthalmology, 2022-01, Vol.25 (1), p.62-72</ispartof><rights>2021 American College of Veterinary Ophthalmologists.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3256-6dba4e55c43cb415cf4240ef75b82d044e6d3a37f31206f4bfb25cbfbaffc1a43</citedby><cites>FETCH-LOGICAL-c3256-6dba4e55c43cb415cf4240ef75b82d044e6d3a37f31206f4bfb25cbfbaffc1a43</cites><orcidid>0000-0002-8922-2886</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34240563$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dower, Nathalie Moro Bassil</creatorcontrib><creatorcontrib>Ribeiro, Alexandre Pinto</creatorcontrib><creatorcontrib>Gomes, Lianna Ghisi</creatorcontrib><creatorcontrib>Cássia Martini, Andresa</creatorcontrib><creatorcontrib>Taques, Isis Indaiara Gonçalves Granjeiro</creatorcontrib><creatorcontrib>Almeida, Sayanne Luns Hatum</creatorcontrib><creatorcontrib>Silva, Mayara Inácio Vincenzi</creatorcontrib><creatorcontrib>Aguiar, Daniel Moura</creatorcontrib><title>Concentrations of tissue inhibitor of matrix metalloproteinase‐1 and hyaluronic acid in canine amniotic membranes cryopreserved for different time points and its effects in dogs with complicated corneal ulcers</title><title>Veterinary ophthalmology</title><addtitle>Vet Ophthalmol</addtitle><description>Objectives
To determine the concentrations of total protein (TP), tissue inhibitor of matrix metalloproteinase‐1 (TIMP‐1) and hyaluronic acid (HA) in amniotic membranes (AMs) harvested from placentas of bitches of different ages and cryopreserved for different time points. The outcomes of complicated corneal defects of dogs repaired with AMs stored for the same time points were also evaluated.
Procedures
Ten cryopreserved canine AMs were stored for short term (2–50 days), middle term (92–210 days), or long term (256–357 days). TP was quantified by Bradford’s test, whereas TIMP‐1 and HA were quantified by ELISA. Twenty‐one dogs that had an AM transplantation to restore deep or perforating corneal wounds were selected.
Results
TIMP‐1 levels were lower in AMs cryopreserved for middle term (p = .02) and long term (p = .0009), when compared to AMs stored for short term. TP (p = .39) and HA (p = .18) concentrations in AMs did not differ among the storage time. TIMP‐1 concentration in AMs correlated with storage time (R = −.65, p < .0001), while TP (R = −.33, p = .07) and HA concentrations did not (R = −.15, p = .41). The age of donors did not correlate with the components evaluated in the AMs. Corneal defects repaired with AMs stored for short term healed sooner than the ones repaired with AMs stored for middle (p < .01) and long term (p = .02). Additionally, TIMP‐1 levels in AMs correlated negatively with the epithelization time (R = −.62, p = .002). Graft opacity was severe in 55% of cases. However, the HA levels in AMs correlated negatively with the opacification score (R = −.47, p = .03). Vision was observed in more patients who presented deep ulcers and descemetoceles, than in the ones with perforations (p = .004).
Conclusions
TIMP‐1 concentration in canine AMs significantly decreased over a year storage time, while TP and HA concentrations did not change during the same period. The age of donors did not correlate with the components evaluated in the AMs. Complicated corneal defects repaired with AMs cryopreserved for short term healed sooner and tended to be less opaque; however, satisfactory to optimal outcomes were achieved even in the eyes repaired with AMs stored for up to a year.</description><subject>Amnion - transplantation</subject><subject>Animals</subject><subject>canine amnion</subject><subject>corneal perforation</subject><subject>Corneal Ulcer - veterinary</subject><subject>descemetocele</subject><subject>Dog Diseases - surgery</subject><subject>Dogs</subject><subject>Female</subject><subject>graft age</subject><subject>Hyaluronic Acid</subject><subject>Matrix Metalloproteinase 1</subject><subject>Pregnancy</subject><subject>Tissue Inhibitor of Metalloproteinase-1</subject><subject>total protein</subject><issn>1463-5216</issn><issn>1463-5224</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kUtuFDEQhlsIREJgwQWQl7CYxO8JSzQKDylSWADbltsuM4X8aGx3wuw4AnfjBpwETyZkhxcuq_zXV3b9w_Cc0VPW19l1nk8Zf830g-GYSS1WinP58P7M9NHwpNZvlFKh6PrxcCQkl1RpcTz83uRkIbViGuZUSfakYa0LEExbnLDlss9F0wr-IBGaCSHPJTfAZCr8-fmLEZMc2e5MWEpOaImx6Ho1sSZhAmJiwtx6PkKciklQiS27zoAK5Roc8b2FQ--h9Hf07hHInDG1egvGHqFf2h471OWvldxg2xKb4xzQmtYRNpcEJpAlWCj16fDIm1Dh2V08GT6_vfi0eb-6vHr3YfPmcmUFV3ql3WQkKGWlsJNkyvr9VMCv1XTOHZUStBNGrL1gnGovJz9xZftuvLfMSHEyvDxw-zy-L1DbGLFaCKF_Mi915EpRrs-F5l366iC1JddawI9zwWjKbmR03Hs4dg_HWw-79sUddpkiuHvlP9O64OwguMEAu_-Txi9XHw_IvxY9roQ</recordid><startdate>202201</startdate><enddate>202201</enddate><creator>Dower, Nathalie Moro Bassil</creator><creator>Ribeiro, Alexandre Pinto</creator><creator>Gomes, Lianna Ghisi</creator><creator>Cássia Martini, Andresa</creator><creator>Taques, Isis Indaiara Gonçalves Granjeiro</creator><creator>Almeida, Sayanne Luns Hatum</creator><creator>Silva, Mayara Inácio Vincenzi</creator><creator>Aguiar, Daniel Moura</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8922-2886</orcidid></search><sort><creationdate>202201</creationdate><title>Concentrations of tissue inhibitor of matrix metalloproteinase‐1 and hyaluronic acid in canine amniotic membranes cryopreserved for different time points and its effects in dogs with complicated corneal ulcers</title><author>Dower, Nathalie Moro Bassil ; Ribeiro, Alexandre Pinto ; Gomes, Lianna Ghisi ; Cássia Martini, Andresa ; Taques, Isis Indaiara Gonçalves Granjeiro ; Almeida, Sayanne Luns Hatum ; Silva, Mayara Inácio Vincenzi ; Aguiar, Daniel Moura</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3256-6dba4e55c43cb415cf4240ef75b82d044e6d3a37f31206f4bfb25cbfbaffc1a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amnion - transplantation</topic><topic>Animals</topic><topic>canine amnion</topic><topic>corneal perforation</topic><topic>Corneal Ulcer - veterinary</topic><topic>descemetocele</topic><topic>Dog Diseases - surgery</topic><topic>Dogs</topic><topic>Female</topic><topic>graft age</topic><topic>Hyaluronic Acid</topic><topic>Matrix Metalloproteinase 1</topic><topic>Pregnancy</topic><topic>Tissue Inhibitor of Metalloproteinase-1</topic><topic>total protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dower, Nathalie Moro Bassil</creatorcontrib><creatorcontrib>Ribeiro, Alexandre Pinto</creatorcontrib><creatorcontrib>Gomes, Lianna Ghisi</creatorcontrib><creatorcontrib>Cássia Martini, Andresa</creatorcontrib><creatorcontrib>Taques, Isis Indaiara Gonçalves Granjeiro</creatorcontrib><creatorcontrib>Almeida, Sayanne Luns Hatum</creatorcontrib><creatorcontrib>Silva, Mayara Inácio Vincenzi</creatorcontrib><creatorcontrib>Aguiar, Daniel Moura</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dower, Nathalie Moro Bassil</au><au>Ribeiro, Alexandre Pinto</au><au>Gomes, Lianna Ghisi</au><au>Cássia Martini, Andresa</au><au>Taques, Isis Indaiara Gonçalves Granjeiro</au><au>Almeida, Sayanne Luns Hatum</au><au>Silva, Mayara Inácio Vincenzi</au><au>Aguiar, Daniel Moura</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Concentrations of tissue inhibitor of matrix metalloproteinase‐1 and hyaluronic acid in canine amniotic membranes cryopreserved for different time points and its effects in dogs with complicated corneal ulcers</atitle><jtitle>Veterinary ophthalmology</jtitle><addtitle>Vet Ophthalmol</addtitle><date>2022-01</date><risdate>2022</risdate><volume>25</volume><issue>1</issue><spage>62</spage><epage>72</epage><pages>62-72</pages><issn>1463-5216</issn><eissn>1463-5224</eissn><abstract>Objectives
To determine the concentrations of total protein (TP), tissue inhibitor of matrix metalloproteinase‐1 (TIMP‐1) and hyaluronic acid (HA) in amniotic membranes (AMs) harvested from placentas of bitches of different ages and cryopreserved for different time points. The outcomes of complicated corneal defects of dogs repaired with AMs stored for the same time points were also evaluated.
Procedures
Ten cryopreserved canine AMs were stored for short term (2–50 days), middle term (92–210 days), or long term (256–357 days). TP was quantified by Bradford’s test, whereas TIMP‐1 and HA were quantified by ELISA. Twenty‐one dogs that had an AM transplantation to restore deep or perforating corneal wounds were selected.
Results
TIMP‐1 levels were lower in AMs cryopreserved for middle term (p = .02) and long term (p = .0009), when compared to AMs stored for short term. TP (p = .39) and HA (p = .18) concentrations in AMs did not differ among the storage time. TIMP‐1 concentration in AMs correlated with storage time (R = −.65, p < .0001), while TP (R = −.33, p = .07) and HA concentrations did not (R = −.15, p = .41). The age of donors did not correlate with the components evaluated in the AMs. Corneal defects repaired with AMs stored for short term healed sooner than the ones repaired with AMs stored for middle (p < .01) and long term (p = .02). Additionally, TIMP‐1 levels in AMs correlated negatively with the epithelization time (R = −.62, p = .002). Graft opacity was severe in 55% of cases. However, the HA levels in AMs correlated negatively with the opacification score (R = −.47, p = .03). Vision was observed in more patients who presented deep ulcers and descemetoceles, than in the ones with perforations (p = .004).
Conclusions
TIMP‐1 concentration in canine AMs significantly decreased over a year storage time, while TP and HA concentrations did not change during the same period. The age of donors did not correlate with the components evaluated in the AMs. Complicated corneal defects repaired with AMs cryopreserved for short term healed sooner and tended to be less opaque; however, satisfactory to optimal outcomes were achieved even in the eyes repaired with AMs stored for up to a year.</abstract><cop>England</cop><pmid>34240563</pmid><doi>10.1111/vop.12916</doi><tpages>0</tpages><orcidid>https://orcid.org/0000-0002-8922-2886</orcidid></addata></record> |
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| ispartof | Veterinary ophthalmology, 2022-01, Vol.25 (1), p.62-72 |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Amnion - transplantation Animals canine amnion corneal perforation Corneal Ulcer - veterinary descemetocele Dog Diseases - surgery Dogs Female graft age Hyaluronic Acid Matrix Metalloproteinase 1 Pregnancy Tissue Inhibitor of Metalloproteinase-1 total protein |
| title | Concentrations of tissue inhibitor of matrix metalloproteinase‐1 and hyaluronic acid in canine amniotic membranes cryopreserved for different time points and its effects in dogs with complicated corneal ulcers |
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