Phylogeny and evolution of mycophagy in the Zygothrica genus group (Diptera: Drosophilidae)

• Published in: Molecular phylogenetics and evolution 2021-10, Vol.163, p.107257-107257, Article 107257
• Main Authors: Zhang, Yang, Katoh, Takehiro K., Finet, Cédric, Izumitani, Hiroyuki F., Toda, Masanori J., Watabe, Hide-aki, Katoh, Toru
• Format: Article
• Language: English
• Subjects: Ancestral state reconstruction; Food habits; Hirtodrosophila; Mycodrosophila; Paramycodrosophila; Zygothrica
• ISSN: 1055-7903; 1095-9513
• DOI: 10.1016/j.ympev.2021.107257

[Display omitted] •The Zygothrica genus group is monophyletic and it has a sister relationship with the genus Dichaetophora.•Both Hirtodrosophila and Zygothrica emerged as non-monophyletic while Mycodrosophila and Paramycodrosophila emerged as monophyletic.•The MRCA of the subgenus Drosophila as a generalist depended on fermenting fruits and decayed fungi.•The MRCA of the Zygothrica genus group as a specialist preferred fresh fungal fruiting bodies. Despite numerous phylogenetic studies on the family Drosophilidae, relationships among some important lineages are still poorly resolved. An example is the equivocal position of the Zygothrica genus group that is mostly comprised of the mycophagous genera Hirtodrosophila, Mycodrosophila, Paramycodrosophila, and Zygothrica. To fill this gap, we conducted a phylogenetic analysis by assembling a dataset of 24 genes from 92 species, including 42 species of the Zygothrica genus group mainly from the Palearctic and Oriental regions. The resulting tree shows that the Zygothrica genus group is monophyletic and places it as the sister to the genus Dichaetophora, and the clade Zygothrica genus group + Dichaetophora is sister to the Siphlodora + Idiomyia/Scaptomyza clade. Within the Zygothrica genus group, the genera Mycodrosophila and Paramycodrosophila are both recognized as monophyletic, while neither the genus Zygothrica nor Hirtodrosophila is monophyletic. We also used this phylogenetic tree to investigate the evolution of mycophagy by reconstructing ancestral food habit in the Drosophilidae. We found that fungus-feeding habit has been gained independently in two lineages. The most recent common ancestor (MRCA) of the subgenus Drosophila was estimated to have acquired mycophagy by expanding its ancestral feeding niche on fermenting fruits to decayed fungi, while the MRCA of the Zygothrica genus group shifted its niche from fruits to fungi as a specialist probably preferring fresh fruiting bodies.