Induction of ischemic tolerance by remote perconditioning or postconditioning as neuroprotective strategy for spinal cord motor neurons

• Published in: Life sciences (1973) 2021-10, Vol.283, p.119789-119789, Article 119789
• Main Authors: Danková, Marianna, Domoráková, Iveta, Fagová, Zuzana, Stebnický, Milan, Mechírová, Eva
• Format: Article
• Language: English
• Subjects: Aorta; Compression; Conditioning; Damage detection; Degeneration; Hsp70 protein; Ischemia; Motor neurons; Neurons; Neuroprotection; Occlusion; Rabbit; Remote perconditioning; Remote postconditioning; Renal artery; Reperfusion; Spinal cord
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2021.119789

The study is focused on the investigation of the mechanisms leading to ischemic tolerance acquisition in the spinal cord neurons via application of non-invasive method of remote conditioning. We have verified the possibility of neuroprotection of spinal cord in rabbit by using remote perconditioning (PerC) applied during last 12 min of spinal cord ischemia (SC-ischemia) or postconditioning (PostC) applied after 1st (early) or 3rd (late) h of reperfusion. Spinal cord ischemia was induced by occlusion of the aorta below the left renal artery for 20 min. Reperfusion period was 24 or 72 h. Remote conditioning was induced by compression of left forelimb with a tourniquet in 3 cycles of 2 min of ischemia, each followed by 2 min of reperfusion. Damaged neurons were detected by Fluoro Jade B method and the modified Tarlov score was used for functional assessment. The remote conditioning significantly attenuated degeneration of motor neurons in all remote conditioned groups versus both SC-ischemia groups. We detected significant changes in number of Hsp70 positive motor neurons. At 72time point, in the group with remote late PostC we observed significant increase (p