Serratia marcescens SCH909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms

ABSTRACT Serratia marcescens SCH909 is a multidrug resistant strain isolated in 1988 harboring three class 1 integrons. We wondered if these integrons were retained over time and if there were other antimicrobial resistant determinants contributing to its multidrug resistant profile. Genomic analysi...

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Bibliographic Details
Published in:FEMS microbiology letters 2021-07, Vol.368 (14), p.1
Main Authors: Gambino, Anahí S, Déraspe, Maxime, Álvarez, Verónica E, Quiroga, María Paula, Corbeil, Jacques, Roy, Paul H, Centrón, Daniela
Format: Article
Language:English
Subjects:
Analysis
Antimicrobial agents
Antimicrobial resistance
Arsenic
Bacteria
Cassettes
Drug resistance
Drug resistance in microorganisms
Genes
Genetic aspects
Genomic analysis
Genomic islands
Hospitals
Identification and classification
Insertion sequences
Microbiology
Multidrug resistance
Nosocomial infection
Pathogenic microorganisms
Plasmids
Serratia marcescens
Transposons
Variable region
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Summary:ABSTRACT Serratia marcescens SCH909 is a multidrug resistant strain isolated in 1988 harboring three class 1 integrons. We wondered if these integrons were retained over time and if there were other antimicrobial resistant determinants contributing to its multidrug resistant profile. Genomic analysis showed a fourth multidrug resistance integron, a Tn7 transposon with dfrA1-sat2-ybeA-ybfA-ybfB-ybgA gene cassettes in the variable region. Insertion sequences were involved in the genesis of novel composite transposons in the L4 subtype plasmid pSCH909, such as Tn6824 carrying an arsenic regulon and two head to head class 1 integrons surrounded by two complete IS1. Remarkably, a novel chromosomal genomic island, SmaR, was identified, closely related to Multiple Antimicrobial Resistance Regions (MARR), usually found in AbaR0-type and AbGRI2-0 from global clones of Acinetobacter baumannii, and in M-type plasmids circulating in Enterobacteriaceae. Maintenance studies showed that the three class 1 integrons were maintained over 1 month without antimicrobial pressure. Since S. marcescens is considered a relevant nosocomial pathogen that can have a wide range of niches – human, plant, animal, soil and inanimate surfaces, our findings support the ability of this species to capture, maintain and spread a broad variety of antimicrobial resistance elements. Genomic plasticity of Serratia marcescens capable of hosting multiple antibiotic resistance genes.
ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fnab086