Mechanism of a Disassembly-Driven Sensing System Studied by Stopped-Flow Kinetics

We carried out steady-state and stopped-flow photophysical measurements to determine the kinetics of a discrete disassembly driven turn-on fluorescent system. On and off rates for both DimerDye1 assembly and nicotine binding were determined. Relative rates for these competing processes provide insig...

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Bibliographic Details
Published in:Journal of organic chemistry 2021-08, Vol.86 (15), p.10782-10787
Main Authors: Gallo, Cara, Thomas, Suma S, Selinger, Allison J, Hof, Fraser, Bohne, Cornelia
Format: Article
Language:English
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Summary:We carried out steady-state and stopped-flow photophysical measurements to determine the kinetics of a discrete disassembly driven turn-on fluorescent system. On and off rates for both DimerDye1 assembly and nicotine binding were determined. Relative rates for these competing processes provide insight on how this system can be optimized for sensing applications. Kinetics studies in artificial saliva showed that moving to more complex media has minimal effects on the sensing ability of the system.
ISSN:0022-3263
1520-6904
DOI:10.1021/acs.joc.1c00959