Effects of Kumaizasa ( Sasa senanensis ) Leaf Extract on Innate Immune Regulation in HEK293 Cells and Macrophages

• Published in: Anticancer research 2021-08, Vol.41 (8), p.4093-4100
• Main Authors: Sato, Keisuke, Tatsunami, Ryosuke, Nakata, Akifumi, Komatsu, Ken-Ichi, Harakawa, Shinji, Nedachi, Takaki, Haketa, Kaoru, Inagawa, Hiroyuki, Wakame, Koji
• Format: Article
• Language: English
• Subjects: Cell lines; Gene expression; IL-1β; Immunity; Immunoregulation; Inflammation; Innate immunity; Interleukin 8; Leaves; Lipopolysaccharides; Macrophages; Plant extracts; Proteins; Signal transduction; Tumor necrosis factor-α
• ISSN: 0250-7005; 1791-7530
• DOI: 10.21873/anticanres.15212

Background/Aim: We investigated the effect of Kumaizasa leaf extract (KLE) on innate immunity using the HEK293 and RAW 264.7 cell lines. Materials and Methods: KLE, lipopolysaccharides (LPS), or KLE with LPS were added to RAW 264.7 cells. The TNF-α and IL-1β mRNA expression was then quantified. The expression of MAPKs, NFĸB, TNF-α and IL-1β proteins was also quantified. In addition, KLE was added to HEK293 cells and the IL-8 concentration was measured. Results: In RAW 264.7 cells, KLE increased the levels of TNF-α and IL-1β mRNA. By contrast, when KLE and LPS were added to RAW 264.7 cells, the increase in TNF-α and IL-1β mRNA was ameliorated. Similarly, the expression of JNK and ERK proteins was reduced. The addition of KLE to HEK293 cells induced IL-8 production. Conclusion: Based on these results, a KLE-mediated mechanism may regulate immunity by suppressing the expression of JNK and ERK, which are involved in inflammatory signal transduction.