Co-upgrading of ethanol-assisted depolymerized lignin: A new biological lignin valorization approach for the production of protocatechuic acid and polyhydroxyalkanoic acid

[Display omitted] •Ethanol-assisted depolymerized lignin (EDL) was obtained using ethanol as the solvent.•Effective co-utilization of ethanol and EDL was conducted using P. putida KT2440.•A biological co-upgrading strategy using EDL as the feedstock was demonstrated.•Utilization of formaldehyde, a b...

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Bibliographic Details
Published in:Bioresource technology 2021-10, Vol.338, p.125563-125563, Article 125563
Main Authors: Nguyen, Linh Thanh, Tran, My Ha, Lee, Eun Yeol
Format: Article
Language:English
Subjects:
aldehyde dehydrogenase
biobased products
Co-upgrading
depolymerization
ethanol
Ethanol utilization
formaldehyde
lignin
Lignin valorization
Polyhydroxyalkanoic acid
Protocatechuic acid
Pseudomonas putida
solvents
technology
value added
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Summary:[Display omitted] •Ethanol-assisted depolymerized lignin (EDL) was obtained using ethanol as the solvent.•Effective co-utilization of ethanol and EDL was conducted using P. putida KT2440.•A biological co-upgrading strategy using EDL as the feedstock was demonstrated.•Utilization of formaldehyde, a bypass product, promoted cell growth in EDL.•Biological production of PCA and biodegradable PHA from EDL has been demonstrated. This study presents a promising biological co-upgrading of ethanol-assisted depolymerized lignin (EDL) into protocatechuic acid (PCA) and polyhydroxyalkanoic acid (PHA) without any separation process. A depolymerized alkali lignin containing various G-lignin-type monomers at a concentration of 77 mg/mL was used for co-upgrading. An engineered Pseudomonas putida KT2440 strain was constructed by knocking out the protocatechuate 3, 4-dioxygenase, expression of the formaldehyde utilization pathway, and the expression of aldehyde dehydrogenase to enhance the efficiency of the ethanol utilization pathway. The growth and production of value-added bioproducts have been promoted by the utilization of formaldehyde, resulted in 6.73 ± 0.26 mg/L of PCA with a 17.5% (w/w) yield of total lignin monomers, and 303.66 ± 26.75 mg/L of PHA with 21.26% (w/w) of dry cell weight from 0.5 mL EDL. Moreover, the ethanol solvent used for lignin depolymerization was also utilized along with depolymerized lignin for co-upgrading to value-added products.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2021.125563