Inhibited HDAC3 promotes microRNA-376c-3p to suppress malignant phenotypes of gastric cancer cells by reducing WNT2b

Our study aims to identify the impact of histone deacetylase 3 (HDAC3) and microRNA-376c-3p (miR-376c-3p) on gastric cancer (GC) by targeting wingless-type MMTV integration site family member 2b (WNT2b). Levels of miR-376c-3p, HDAC3 and WNT2b were assessed. GC cells were treated with altered HDAC3 o...

Full description

Saved in:
Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2021-11, Vol.113 (6), p.3512-3522
Main Authors: Zhang, Lan, Liu, Fanglin, Meng, Zhixing, Luo, Qingyue, Pan, Daojing, Qian, Yihua
Format: Article
Language:English
Subjects:
Apoptosis
Cell Proliferation - genetics
Gastric cancer
Glycoproteins - genetics
Histone deacetylase 3
Histone Deacetylases - genetics
Histone Deacetylases - metabolism
Humans
MicroRNA-376c-3p
MicroRNAs - genetics
MicroRNAs - metabolism
Phenotype
Proliferation
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Wingless-type MMTV integration site family member 2b
Wnt Proteins - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Our study aims to identify the impact of histone deacetylase 3 (HDAC3) and microRNA-376c-3p (miR-376c-3p) on gastric cancer (GC) by targeting wingless-type MMTV integration site family member 2b (WNT2b). Levels of miR-376c-3p, HDAC3 and WNT2b were assessed. GC cells were treated with altered HDAC3 or miR-376c-3p to evaluate their biological functions, and rescue experiment was performed to assess the effect of WNT2b on GC cells. The tumor growth in vivo was observed. HDAC3 and WNT2b were up-regulated while miR-376c-3p was reduced in GC tissues and cell lines. The inhibited HDAC3 or elevated miR-376c-3p could restrain malignant behaviors of GC cells in vitro, and also suppress the xenograft growth. WNT2b silencing reduced the effect of miR-376c-3p inhibition while WNT2b overexpression mitigated that of miR-376c-3p promotion on GC cell growth. Inhibiting HDAC3 promotes miR-376c-3p to suppress malignant phenotypes of GC cells via reducing WNT2b, thereby restricting GC development. •HDAC3 and WNT2b are elevated while miR-376c-3p is reduced in GC tissues.•Inhibited HDAC3/elevated miR-376c-3p suppress WNT2b expression in GC cells.•Inhibited HDAC3/elevated miR-376c-3p reduce GC cell growth.•Inhibited HDAC3/elevated miR-376c-3p accelerate GC cell apoptosis.•WNT2b is a direct target gene of miR-376c-3p.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2021.07.018