Cloning, expression and serodiagnostic potential of HSP70 of Taenia multiceps in sheep

[Display omitted] •The study was designed to determine the effectiveness of r-TmHSP70 antigen in the serodiagnosis of sheep coenurosis.•Sensitivity and specificity (Se/Sp) of the r-TmHSP70 antigen were 100 % in Western blot.•The Se/Sp values of the r-TmHSP70 were 66.6 % and 52.6 %, respectively in E...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 2021-09, Vol.245, p.111397-111397, Article 111397
Main Authors: Gunyakti Kilinc, Seyma, Simsek, Sami, Celik, Figen, Kesik, Harun Kaya
Format: Article
Language:English
Subjects:
Animals
Cloning
Cloning, Molecular
Goat Diseases
Goats
HSP70
Serologic Tests
Sheep
Taenia - genetics
Taenia multiceps
Taeniasis
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Summary:[Display omitted] •The study was designed to determine the effectiveness of r-TmHSP70 antigen in the serodiagnosis of sheep coenurosis.•Sensitivity and specificity (Se/Sp) of the r-TmHSP70 antigen were 100 % in Western blot.•The Se/Sp values of the r-TmHSP70 were 66.6 % and 52.6 %, respectively in ELISA.•567 (46.97 %) of the 1207 sera samples analyzed were found to be positive by ELISA. The aim of this study was to determine the efficacy of the recombinant protein expressed by the T. multiceps HSP70 gene in the immunodiagnosis of sheep coenurosis. Specific primers were designed to amplify the gene encoding HSP70 from the whole genome sequence of T. multiceps, mRNA was isolated from C. cerebralis cysts obtained from an infected sheep’s brain, and then, cDNA was isolated. The gene region related to the designed primers was amplified by PCR, and the obtained product was cloned and expressed. The specificity of the recombinant protein purified afterwards was demonstrated by Western Blot using known positive and negative sheep sera. Additionally, the efficiency of the recombinant protein in ELISA was determined with sera collected from 1207 sheep. Finally, the sensitivity and specificity values ​of the recombinant TmHSP70 antigen in the Western Blot were 100 %, while the sensitivity and specificity rates of the ELISA were 66.6 % and 52.6 %, respectively. At the same time, 567 (46.97 %) of the 1207 analyzed serum samples were found to be positive by ELISA. In conclusion, the Western Blot technique had a higher quality than ELISA in detecting the TmHSP70 antigen for the serodiagnosis of sheep coenurosis.
ISSN:0166-6851
1872-9428
DOI:10.1016/j.molbiopara.2021.111397