Functional analysis of pde gene and its role in the pathogenesis of Xanthomonas oryzae pv. oryzicola

• Published in: Infection, genetics and evolution genetics and evolution, 2021-10, Vol.94, p.105008-105008, Article 105008
• Main Authors: Wan, Xiaoyan, Yang, Jun, Ahmed, Waqar, Liu, Qi, Wang, Yanfang, Wei, Lanfang, Ji, Guanghai
• Format: Article
• Language: English
• Subjects: Bacterial leaf streak; Biofilms; c-di-GMP; Flagella - physiology; Gene Deletion; Genes, Bacterial; pde gene; Plant Diseases - microbiology; Polysaccharides, Bacterial - biosynthesis; Polysaccharides, Bacterial - genetics; Virulence; Xanthomonas - genetics; Xanthomonas - pathogenicity; Xanthomonas oryzae pv. oryzicola
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2021.105008

Bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola (Xoc) is a devastating disease of rice worldwide, including China. The second messenger c-di-GMP plays an important role in the transduction of intercellular signals. However, little is known about the function of EAL domain protein in c-di-GMP that regulates the virulence in Xoc. In this study, the function of EAL domain protein encoded by pde (FE36_09715) gene in the regulation of c-di-GMP was investigated. Results of this study, showed that the deletion of pde gene led to a significant reduction in the virulence of Xoc and was positively related to the reduction of exopolysaccharides production, biofilm formation, and flagellar motility. However, these significantly impaired properties from the ∆pde mutant strain were partially recovered in the complementary strain. In addition, the deletion of pde gene in Xoc strain YM15 had no visible effect on the colony morphology, amylase, and protease activities of Xoc. It is concluded that, as a regulator for the c-di-GMP level, the pde gene plays an important role in partial biological processes in Xoc and is essential for its virulence. •Bacterial leaf streak caused by Xanthomonas oryzae pv. oryzicola is a devastating disease of rice.•pde gene plays an important role in the virulence of Xanthomonas oryzae pv. oryzicola.•pde gene is positively related to reductions of exopolysaccharides production, biofilm formation, and flagellar motility.•Deletion of pde gene had no visible effect on colony morphology, amylase, and protease activities.