Molecular characterization of non-specific immune genes of endangered golden mahseer (Tor putitora) and their expression during embryonic and larval development

• Published in: Fish & shellfish immunology 2021-11, Vol.118, p.119-146
• Main Authors: Akhtar, M.S., Tripathi, Priyanka H., Rajesh, Manchi, Pandey, Anupam, Kamalam, Biju Sam, Ciji, A.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Cyprinidae - genetics; Cyprinidae - immunology; Cytokines; Gene expression; Golden mahseer; Hepcidin; Immune genes; Interleukin-10; Molecular characterization; Ontogeny; RNA, Messenger; Tumor Necrosis Factor-alpha
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2021.07.016

The present study was undertaken to characterize and analyze the expression of non-specific immune genes to get an insight into the early immune status of endangered golden mahseer. In this study, the full-length mRNA sequence of IFNγ, TNFα, C3, and IL10 was 927, 1409, 5125 and 1177 bp with an ORF of 558, 765, 4938, and 540 bp, encoding a putative protein of 185, 254, 1645, and 179 amino acid residues, respectively. The deduced amino acid sequences of these genes shared highly conserved structures with those from other cyprinids. Ontogenic real-time qPCR results indicated that expression of IFNγ and TNFα was lower until the morula stage and increased from blastula stage and found maximum at the organogenesis stage. Expression of the C3 gene was lower until the gastrula stage, followed by a linear increase from organogenesis to the pre-metamorphosis stage. The expression of IL10 was significantly lower during early developmental stages (till gastrula stage) and reached maximum at organogenesis. The level of IL1β was found maximum in unfertilized eggs and remained elevated till the morula stage. TLR4 expression remained lower during the initial developmental stages and reached the maximum at the organogenesis stage. The expression level of defensin1 was substantially low until the organogenesis stage. In comparison, hepcidin1 was found considerably high until the blastula stage and remained significantly lower during later stages of development. Overall, the data generated improves knowledge on the immune status of endangered golden mahseer during embryonic and larval development, which may help develop effective immunomodulatory interventions during nursery rearing of golden mahseer to produce fry with better fitness. •Molecular characterization of IFNγ, TNFα, C3, and IL10 in Tor putitora is reported.•IFNγ, TNFα, C3, and IL10 encode a putative protein of 185, 254, 1645, and 179 amino acid residues, respectively.•The relative expression of eight immune genes was differentially regulated during the ontogenic development of T. putitora.•The onset of non-specific immuno-competence in T. putitora occurs even before hatching.•The relative expression of few immune genes was observed in unfertilized eggs.