LC-MS/MS targeting analysis of terpenoid metabolism in Carya cathayensis at different developmental stages

• Published in: Food chemistry 2022-01, Vol.366, p.130583-130583, Article 130583
• Main Authors: Zhang, Yuan-Yuan, Elam, Elnur, Ni, Zhi-Jing, Zhang, Fan, Thakur, Kiran, Wang, Shaoyun, Zhang, Jian-Guo, Wei, Zhao-Jun
• Format: Article
• Language: English
• Subjects: Carya cathayensis; Co-expression network; Metabolites; Terpenoid quinone
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2021.130583

[Display omitted] •The terpenoid metabolism in four different development stages of C. cathayensis was elucidated.•Six terpenoid metabolites and 86 DEGs related to terpenoquinone metabolism were identified.•Based on RNA-Seq analysis, 679 genes of terpenoid biosynthesis were differently expressed.•The metabolome and transcriptome data showed that terpenoquinone accumulated in the early stages. Terpenoid metabolism at different developmental stages of Carya cathayensis was elucidated based on LC-MS/MS analysis and multi-omics. Terpenoid metabolites 2-hydroxy-1,4-naphoquinone and 3-hydroxybenzoic acid reached the maximum at 105 days after pollination (DAP) (P2 stage). To reveal the complex mechanism of C. cathayensis embryogenesis in relation to terpenoid metabolites (90–165 DAP), a metabolomic and transcriptional co-expression analysis was conducted. Based on RNA-Seq analysis, 679 genes of 1144 terpenoid biosynthesis were differentially expressed. Six terpenoid metabolites and 86 differentially expressed genes related to terpenoquinone metabolism were identified. Comprehensive analysis of metabolome and transcriptional data revealed that terpenoquinone accumulated in the early phase was active in the later phase. Overall, we profiled the transcriptome and metabolome changes in C. cathayensis during the developmental phase to investigate the metabolic pathways and candidate genes underlying the changes at different growth stages.