Effective Model of Food Allergy in Mice Sensitized with Ovalbumin and Freud’s Adjuvant

• Published in: Bulletin of experimental biology and medicine 2021-07, Vol.171 (3), p.352-356
• Main Authors: Zhang, Y., Liu, J. Y., Shao, J. W., Luo, Q. Q., Zhang, Y. Q., Song, G., Wang, C. Y., Zhao, S. Y., Wan, C., Du, X. H., Xu, L. Z.
• Format: Article
• Language: English
• Subjects: Albumin; Analysis; Anaphylaxis; Anaphylaxis - chemically induced; Anaphylaxis - genetics; Anaphylaxis - immunology; Anaphylaxis - pathology; Animals; B cells; Biomarkers - metabolism; Biomedical and Life Sciences; Biomedicine; Cell Biology; Chymase; Chymases - genetics; Chymases - immunology; Colon; Colon - immunology; Colon - pathology; Duodenum; Duodenum - immunology; Duodenum - pathology; Egg Hypersensitivity - genetics; Egg Hypersensitivity - immunology; Egg Hypersensitivity - pathology; Extracellular Fluid - chemistry; Extracellular Fluid - immunology; Female; Food allergies; Food allergy; Freund's Adjuvant - administration & dosage; Gene Expression; Ileum; Ileum - immunology; Ileum - pathology; Immunoglobulin E; Immunoglobulin E - blood; Immunoglobulin G; Immunoglobulin G - blood; Immunotherapy; Inflammation; Internal Medicine; Jejunum; Jejunum - immunology; Jejunum - pathology; Laboratory Medicine; Mice; Mice, Inbred BALB C; Ovalbumin; Ovalbumin - administration & dosage; Ovalbumin - immunology; Pathology; Proteases; Small intestine
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-021-05226-z

To better explore the pathophysiology of FA and its therapy, we aimed to establish a simple and practicable FA model with Freund’s adjuvant and introduce an easy and reliable laboratory evaluation method for assessment of inflammation in intestinal segments at different anatomical locations. BALB/c mice were sensitized with ovalbumin combined with Freund’s adjuvant. Complete Freund’s adjuvant was chosen for the first sensitization and two weeks later incomplete Freund’s adjuvant was used for a second sensitization. Two weeks later, the sensitized mice were challenged with 50 mg ovalbumin every other day. After the 6 challenge, all mice were assessed for systemic anaphylaxis, and then sacrificed for sample collection. All sensitized mice showed anaphylactic symptoms and markedly increased levels of serum ovalbumin-specific IgE and IgG1. The activity of mast cell protease-1 (mMCPT-1) was significantly increased in the serum and interstitial fluid of the duodenum, jejunum, ileum, and colon. A successful FA model was established, of which inflammation occurred in the duodenum, jejunum, ileum, and colon. This model provides a reliable and simple tool for analysis of the mechanism of FA and methods of immunotherapy. Moreover, combined detection of ovalbumin-specific antibody and local mMCPT-1 levels could potentially be used as the major indicator for assessment of food allergy.