Shortwave-Infrared Fluorescent Molecular Imaging Probes Based on π‑Conjugation Extended Indocyanine Green

Recently, shortwave-infrared (SWIR) fluorescence imaging for the optical diagnostics of diseases has attracted much attention as a new noninvasive imaging modality. For this application, the development of SWIR molecular imaging probes with high biocompatibility is crucial. Although many types of bi...

Full description

Saved in:
Bibliographic Details
Published in:Bioconjugate chemistry 2021-08, Vol.32 (8), p.1541-1547
Main Authors: Swamy, Mahadeva M. M, Murai, Yuta, Monde, Kenji, Tsuboi, Setsuko, Jin, Takashi
Format: Article
Language:English
Subjects:
Biocompatibility
Biomedical materials
Biomolecules
Breast cancer
Breast Neoplasms - diagnostic imaging
Breast Neoplasms - pathology
Cell Line, Tumor
Conjugation
Epidermal growth factor
ErbB Receptors - analysis
ErbB-2 protein
Female
Fluorescence
Fluorescent dyes
Fluorescent Dyes - analysis
Fluorescent Dyes - chemistry
Fluorescent indicators
Food dyes
Growth factors
Humans
Indocyanine Green - analogs & derivatives
Indocyanine Green - analysis
Infrared imaging
Molecular Imaging - methods
Monoclonal antibodies
Optical Imaging - methods
Probes
Receptor, ErbB-2 - analysis
Receptors
Short wave radiation
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recently, shortwave-infrared (SWIR) fluorescence imaging for the optical diagnostics of diseases has attracted much attention as a new noninvasive imaging modality. For this application, the development of SWIR molecular imaging probes with high biocompatibility is crucial. Although many types of biocompatible SWIR fluorescent probes based on organic dyes have been reported, there are no SWIR-emitting molecular imaging probes that can be used for the detection of specific biomolecules in vivo. To apply SWIR-emitting molecular imaging probes to biomedical fields, we developed a biocompatible SWIR fluorescent dye based on π-conjugation extended indocyanine green (ICG), where ICG is the only approved near-infrared dye by the US Food and Drug Administration (FDA) for use in the clinic. Using the π-conjugation extended ICG, we prepared SWIR molecular imaging probes that can be used for in vivo tumor imaging. Herein, we demonstrate noninvasive SWIR fluorescence imaging of human epidermal growth factor receptor 2 (HER2)-positive and epidermal growth factor receptor (EGFR)-positive breast tumors using π-conjugation extended ICG and monoclonal antibody conjugates. The presented π-conjugation extended ICG analog probes will be a breakthrough to apply SWIR fluorescence imaging in biomedical fields.
ISSN:1043-1802
1520-4812
DOI:10.1021/acs.bioconjchem.1c00253