A second capsidless hadakavirus strain with 10 positive-sense single-stranded RNA genomic segments from Fusarium nygamai

A unique capsidless virus with a positive-sense, single-stranded RNA genome (hadakavirus 1, HadV1), a member of the extended picorna-like supergroup, was isolated previously from the phytopathogenic fungus Fusarium oxysporum . Here, we describe the molecular and biological characterisation of a seco...

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Bibliographic Details
Published in:Archives of virology 2021-10, Vol.166 (10), p.2711-2722
Main Authors: Khan, Haris Ahmed, Sato, Yukiyo, Kondo, Hideki, Jamal, Atif, Bhatti, Muhammad Faraz, Suzuki, Nobuhiro
Format: Article
Language:English
Subjects:
Asymptomatic infection
Biomedical and Life Sciences
Biomedicine
Double-stranded RNA
Fusarium
Genomes
Genomics
Infectious Diseases
Medical Microbiology
Nucleotide sequence
Original Article
Phylogenetics
Phytopathogenic fungi
Proteins
Replicative form
Ribonucleic acid
RNA
RNA polymerase
Spores
Virology
Viruses
Zinc finger proteins
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Summary:A unique capsidless virus with a positive-sense, single-stranded RNA genome (hadakavirus 1, HadV1), a member of the extended picorna-like supergroup, was isolated previously from the phytopathogenic fungus Fusarium oxysporum . Here, we describe the molecular and biological characterisation of a second hadakavirus strain from Fusarium nygamai , which has not been investigated in detail previously as a virus host. This virus, hadakavirus 1 strain 1NL (HadV1-1NL), has features similar to the first hadakavirus, HadV1-7n, despite having a different number of segments (10 for HadV1-1NL vs. 11 for HadV1-7n). The 10 genomic RNA segments of HadV1-1NL range in size from 0.9 kb to 2.5 kb. All HadV1-1NL segments show 67% to 86% local nucleotide sequence identity to their HadV1-7n counterparts, whereas HadV1-1NL has no homolog of HadV1-7n RNA8, which encodes a zinc-finger motif. Another interesting feature is the possible coding incapability of HadV1-1NL RNA10. HadV1-1NL was predicted to be capsidless based on the RNase A susceptibility of its replicative form dsRNA. Phenotypic comparison of multiple virus-infected and virus-free single-spore isolates indicated asymptomatic infection by HadV1-1NL. Less-efficient vertical transmission via spores was observed as the infected fungal colonies from which the spores were derived became older, as was observed for HadV1-7n. This study shows a second example of a hadakavirus that appears to have unusual features.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-021-05176-x